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Review
. 2023 Jan 13:14:1076261.
doi: 10.3389/fphys.2023.1076261. eCollection 2023.

Living myocardial slices: Advancing arrhythmia research

Jorik H Amesz  1   2 , Lu Zhang  2 , Bian R Everts  1 , Natasja M S De Groot  2 , Yannick J H J Taverne  1
Affiliations
Review

Living myocardial slices: Advancing arrhythmia research

Jorik H Amesz et al. Front Physiol. .

Abstract

Living myocardial slices (LMS) are ultrathin (150-400 µm) sections of intact myocardium that can be used as a comprehensive model for cardiac arrhythmia research. The recent introduction of biomimetic electromechanical cultivation chambers enables long-term cultivation and easy control of living myocardial slices culture conditions. The aim of this review is to present the potential of this biomimetic interface using living myocardial slices in electrophysiological studies outlining advantages, disadvantages and future perspectives of the model. Furthermore, different electrophysiological techniques and their application on living myocardial slices will be discussed. The developments of living myocardial slices in electrophysiology research will hopefully lead to future breakthroughs in the understanding of cardiac arrhythmia mechanisms and the development of novel therapeutic options.

Keywords: 3D cardiac models; cardiac arrhythmia models; in-vitro electrophysiology; living myocardial slices; translational research.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

FIGURE 1
FIGURE 1
Timeline of major events for living myocardial slices (LMS) in electrophysiological research. 1 Pincus (1933), 2 Burnashev et al. (1990), 3 Halbach et al. (2006), 4 Wang et al. 2015, 5 Fischer et al. (2019), 6 Watson et al. (2019).
FIGURE 2
FIGURE 2
Living myocardial slices are a cardiac arrhythmia model with high degree of in-vivo mimicry to which different electrophysiological recording techniques can be applied. APs action potentials.
See this image and copyright information in PMC

References

    1. Amesz J. H., Groot N. M. S., Langmuur S. J. J., Rooij M. M. M. M., Tiggeloven V. P. C., Bogers A. J. J. C., et al. (2022). Biomimetic cultivation of atrial tissue slices: A novel platform for in-vitro atrial arrhythmia studies. Research Square. 10.21203/rs.3.rs-1799902/v1 - DOI - PMC - PubMed
    1. Bachtel A. D., Gray R. A., Stohlman J. M., Bourgeois E. B., Pollard A. E., Rogers J. M. (2011). A novel approach to dual excitation ratiometric optical mapping of cardiac action potentials with di-4-ANEPPS using pulsed LED excitation. IEEE Trans. Biomed. Eng. 58, 2120–2126. 10.1109/TBME.2011.2148719 - DOI - PMC - PubMed
    1. Barclay C. J. (2005). Modelling diffusive O(2) supply to isolated preparations of mammalian skeletal and cardiac muscle. J. Muscle Res. Cell Motil. 26, 225–235. 10.1007/s10974-005-9013-x - DOI - PubMed
    1. Baxter W. T., Mironov S. F., Zaitsev A. V., Jalife J., Pertsov A. M. (2001). Visualizing excitation waves inside cardiac muscle using transillumination. Biophys. J. 80, 516–530. 10.1016/S0006-3495(01)76034-1 - DOI - PMC - PubMed
    1. Berenfeld O., Efimov I. (2019). Optical mapping. Card. Electrophysiol. Clin. 11, 495–510. 10.1016/j.ccep.2019.04.004 - DOI - PubMed

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