Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 May;60(5):2767-2785.
doi: 10.1007/s12035-023-03216-y. Epub 2023 Jan 30.

lncfos/miR-212-5p/CASP7 Axis-Regulated miR-212-5p Protects the Brain Against Ischemic Damage

Shenghua Li  1 , Xiang Qu  2 , Zhenxiu Qin  2 , Jinggui Gao  2 , Jinpin Li  2 , Jingli Liu  3
Affiliations

lncfos/miR-212-5p/CASP7 Axis-Regulated miR-212-5p Protects the Brain Against Ischemic Damage

Shenghua Li et al. Mol Neurobiol. 2023 May.

Erratum in

Abstract

miR-212-5p has been reported to be involved in many biological processes. However, the role of miR-212-5p in ischemic stroke remains unclear. This study explored the biological role and potential mechanism of miR-212-5p in ischemic stroke by investigating the lncfos/miR-212-5p/CASP7 axis. A total of 32 patients with ischemic stroke and 32 age- and sex-matched healthy controls (HCs) were enrolled in this study. In addition, 336 rats were used in this study. The rats were subjected to middle cerebral artery occlusion (MCAO) and intracerebroventricular injection of a microRNA (miRNA) agomir, a miRNA antagomir, a short hairpin RNA (shRNA) lentiviral vector, or a negative control. The neurological deficit score was calculated; the infarct volume was measured; histopathological assays were performed; the neuronal apoptosis rate was determined; and the lncfos, miR-212-5p, and CASP7 expression levels in the peri-infarct area were assessed. In this study, we found that the expression level of miR-212-5p was significantly downregulated in the peri-infarct area and blood of the MCAO model rats and the blood of patients with ischemic stroke. A double-luciferase experiment showed that CASP7 was a direct target gene of miR-212-5p and that miR-212-5p was a target miRNA of lncfos. Lateral ventricular injection of the miR-212-5p agomir effectively inhibited the apoptosis induced by ischemic brain damage, reduced the infarct volume, attenuated the neurological deficit symptoms, and downregulated the expression of CASP7 in the peri-infarct area of the MCAO model rats. Suppressing lncfos with sh-fos led to the upregulated expression of miR-212-5p and played a neuroprotective role in the rat MCAO models. We concluded that miR-212-5p plays a neuroprotective role in ischemic stroke and that its function is regulated by the lncfos/miR-212-5p/CASP7 axis. Moreover, miR-212-5p may be a potential biomarker and therapeutic target for ischemic stroke.

Keywords: Apoptosis; CASP7; Ischemic stroke; Lncfos; miR-212-5p.

PubMed Disclaimer

Similar articles

See all similar articles

Cited by

References

    1. Chen Y, Wright N, Guo Y, Turnbull I, Kartsonaki C, Yang L et al (2020) Mortality and recurrent vascular events after first incident stroke: a 9-year community-based study of 0.5 million Chinese adults. Lancet Glob Health 8:e580–e590. https://doi.org/10.1016/S2214-109X(20)30069-3 - DOI - PubMed - PMC
    1. Feigin VL, Krishnamurthi RV, Parmar P, Norrving B, Mensah GA, Bennett DA et al (2015) Update on the global burden of ischemic and hemorrhagic stroke in 1990–2013: the GBD 2013 Study. Neuroepidemiology 45:161–176. https://doi.org/10.1159/000441085 - DOI - PubMed
    1. Collaborators, GBDCoD (2017) Global, regional, and national age-sex specific mortality for 264 causes of death, 1980–2016: a systematic analysis for the Global Burden of Disease Study 2016. Lancet 390:1151–1210. https://doi.org/10.1016/S0140-6736(17)32152-9
    1. National Institute of Neurological, D & Stroke rt, PASSG (1995) Tissue plasminogen activator for acute ischemic stroke. N Engl J Med 333:1581–1587. https://doi.org/10.1056/NEJM199512143332401
    1. Kaito M, Araya S, Gondo Y, Fujita M, Minato N, Nakanishi M et al (2013) Relevance of distinct monocyte subsets to clinical course of ischemic stroke patients. PLoS One 8:e69409. https://doi.org/10.1371/journal.pone.0069409 - DOI - PubMed - PMC

MeSH terms

LinkOut - more resources