PASTE: The Way Forward for Large DNA Insertions

Muhammad Arslan Mahmood^{1

2}, Shahid Mansoor^{1

3}

Affiliations

¹ Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE) College Pakistan Institute of Engineering and Applied Sciences, Faisalabad, Pakistan.
² Department of Biological Sciences, University of Sialkot, Sialkot, Pakistan.
³ International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.

Comment

Muhammad Arslan Mahmood et al. CRISPR J. 2023 Feb.

. 2023 Feb;6(1):2-4.

doi: 10.1089/crispr.2023.0001. Epub 2023 Jan 30.

Muhammad Arslan Mahmood^{1

2}, Shahid Mansoor^{1

3}

¹ Agricultural Biotechnology Division, National Institute for Biotechnology and Genetic Engineering (NIBGE) College Pakistan Institute of Engineering and Applied Sciences, Faisalabad, Pakistan.
² Department of Biological Sciences, University of Sialkot, Sialkot, Pakistan.
³ International Center for Chemical and Biological Sciences, University of Karachi, Karachi, Pakistan.

No abstract available

Comment on

Drag-and-drop genome insertion of large sequences without double-strand DNA cleavage using CRISPR-directed integrases.
Yarnall MTN, Ioannidi EI, Schmitt-Ulms C, Krajeski RN, Lim J, Villiger L, Zhou W, Jiang K, Garushyants SK, Roberts N, Zhang L, Vakulskas CA, Walker JA 2nd, Kadina AP, Zepeda AE, Holden K, Ma H, Xie J, Gao G, Foquet L, Bial G, Donnelly SK, Miyata Y, Radiloff DR, Henderson JM, Ujita A, Abudayyeh OO, Gootenberg JS. Yarnall MTN, et al. Nat Biotechnol. 2023 Apr;41(4):500-512. doi: 10.1038/s41587-022-01527-4. Epub 2022 Nov 24. Nat Biotechnol. 2023. PMID: 36424489 Free PMC article.