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Comment
. 2023 Sep;41(9):1206-1207.
doi: 10.1038/s41587-023-01681-3.

Spatial tropism profiling of AAV vectors by ultrasensitive sequential FISH in tissue

No authors listed
Comment

Spatial tropism profiling of AAV vectors by ultrasensitive sequential FISH in tissue

No authors listed. Nat Biotechnol. 2023 Sep.

Abstract

Ultrasensitive sequential fluorescence in situ hybridization (USeqFISH) enables multiplexed detection of the expression of endogenous and exogenous genes delivered by adeno-associated virus (AAV) vectors in intact tissue. USeqFISH provides a spatial map of AAV tropism with high throughput and resolution.

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Figures

Fig. 1
Fig. 1. USeqFISH for in situ cell-type tropism profiling of pooled AAV vectors.
A schematic of the procedure of USeqFISH profiling. vg, vector genomes. © 2023, Jang, M. J. et al., CCBY 4.0.
See this image and copyright information in PMC

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