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Review
. 2023;66(1):43-49.
doi: 10.17116/sudmed20236601143.

[Ways to solve the problem of cross-reactions during immunochromatographic examination of biological objects]

[Article in Russian]
P S Vikman  1 , A S Zhuravleva  1 , O Yu Strelova  1 , A N Grebenyuk  1
Affiliations
Review

[Ways to solve the problem of cross-reactions during immunochromatographic examination of biological objects]

[Article in Russian]
P S Vikman et al. Sud Med Ekspert. 2023.

Abstract
in English, Russian

The purpose of this review is to study the causes of cross-reactions of a number of drugs (mebeverine, phenibut, tropicamide, ramipril, metoprolol, phenylephrine, sertraline, chloropyramine and diphenhydramine) during the preliminary stage of laboratory diagnostics by immunochromatographic method and to propose a possible algorithm for solving this problem. Conducting a hair study in order to identify the fact of the use of psychoactive substances will increase the reliability of analytical diagnostics and reduce the likelihood of false positive results of the analysis. The use of a validated method of enzymatic hydrolysis of hair will eliminate unreliable results of the analysis due to the detection of the native molecule of the toxicant, increase the efficiency and accuracy of the diagnostic procedure.

Обзора — изучить причины перекрестных реакций ряда лекарственных средств (мебеверина, фенибута, тропикамида, рамиприла, метопролола, фенилэфрина, сертралина, хлоропирамина и дифенгидрамина) при проведении предварительного этапа лабораторной диагностики иммунохроматографическим методом и предложить возможный алгоритм решения данной проблемы. Проведение исследования волос с целью выявления факта употребления психоактивных веществ позволит повысить достоверность аналитической диагностики и снизить вероятность получения ложноположительных результатов анализа. Использование валидированной методики ферментативного гидролиза волос позволит исключить недостоверные результаты анализа за счет обнаружения именно нативной молекулы токсиканта, повысить эффективность и точность проведения процедуры диагностики.

Keywords: chloropyramine; cross-reactions; diphenhydramine hydrochloride; enzymatic hydrolysis; hair; immunochromatography; mebeverine; metabolism; metoprolol; phenibut; phenylephrine; ramipril; sertraline; tropicamide.

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