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. 2023:2545:351-372.
doi: 10.1007/978-1-0716-2561-3_18.

Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas

Quinten Bafort  1   2   3 , Lucas Prost  4   5   6 , Eylem Aydogdu  7   8 , Antoine Van de Vloet  7   9   8 , Griet Casteleyn  7   9   8 , Yves Van de Peer  7 , Olivier De Clerck  9
Affiliations

Studying Whole-Genome Duplication Using Experimental Evolution of Chlamydomonas

Quinten Bafort et al. Methods Mol Biol. 2023.

Abstract

In this chapter, we present the use of Chlamydomonas reinhardtii in experiments designed to study the evolutionary impacts of whole genome duplication. We shortly introduce the algal species and depict why it is an excellent model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related Chlamydomonas experiment. These steps include strain selection, ploidy determination, different methods of making diplo- and polyploid Chlamydomonas cells, replication, culturing conditions, preservation, and the ways to quantify phenotypic and genotypic change.

Keywords: Chlamydomonas reinhardtii; Evolve and resequence (E&R); Experimental evolution; Polyploidy; Whole-genome duplication.

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