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. 2023:2545:373-390.
doi: 10.1007/978-1-0716-2561-3_19.

Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza

Tian Wu  1   2 , Annelore Natran  1   2 , Lucas Prost  1   2   3 , Eylem Aydogdu  1   2 , Yves Van de Peer  1 , Quinten Bafort  4   5   6
Affiliations

Studying Whole-Genome Duplication Using Experimental Evolution of Spirodela polyrhiza

Tian Wu et al. Methods Mol Biol. 2023.

Abstract

In this chapter, we present the use of Spirodela polyrhiza in experiments designed to study the evolutionary impact of whole-genome duplication (WGD). We shortly introduce this duckweed species and explain why it is a suitable model for experimental evolution. Subsequently, we discuss the most relevant steps and methods in the design of a ploidy-related duckweed experiment. These steps include strain selection, ploidy determination, different methods of making polyploid duckweeds, replication, culturing conditions, preservation, and the ways to quantify phenotypic and transcriptomic change.

Keywords: Duckweed; Evolve and resequence (E&R); Experimental evolution; Polyploidy; Spirodela polyrhiza; Whole-genome duplication.

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References

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