Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Aug;26(3):651-662.
doi: 10.1007/s10123-023-00331-7. Epub 2023 Feb 4.

Molecular characterization of fungal endophyte diversity isolated from Aconitum heterophyllum: a critically endangered medicinal plant of Kashmir Himalaya

Sabira Hafeez  1   2 , Sabba Yaqoob  1   2 , Aqib Rehman Magray  3   4 , Azra N Kamili  5 , Bashir Ahmad Ganai  6
Affiliations

Molecular characterization of fungal endophyte diversity isolated from Aconitum heterophyllum: a critically endangered medicinal plant of Kashmir Himalaya

Sabira Hafeez et al. Int Microbiol. 2023 Aug.

Abstract

Aconitum heterophyllum is a rare perennial herb from Kashmir Himalayas. Due to its threatened status and dependence on its environment, the plant was examined for any potential endophytes, which is of utmost importance for bioprospection. In the current study, endophytic fungal diversity associated with A. heterophyllum was examined, and 328 fungal isolates were found in the plant's leaf, stem, and root tissues. Twelve (12) endophytic fungal species were identified utilizing, molecular analysis of the nuclear ribosomal DNA Internal Transcribes Spacer (ITS), rLSU, and rSSU sequences. Maximum likelihood analysis was used to determine the phylogenetic connection between each isolate. The genera Arthrinium, Chaetomium, Purpureocillium, Alternaria, Penicillium, Aspergillus, Cladosporium, and Bjerkandera species dominated the ascomycete and basidiomycete fungal endophytes.

Keywords: Endophytes; Kashmir; Medicinal plant; Microbiology; Molecular identification.

PubMed Disclaimer

References

    1. Agut M, Calvo MÁ (2004) In vitro conidial germination in Arthrinium aureum and Arthrinium phaeospermum. Mycopathologia 157(4):363–367 - PubMed - DOI
    1. Allen GC, Flores-Vergara MA, Krasynanski S, Kumar S, Thompson WF (2006) A modified protocol for rapid DNA isolation from plant tissues using cetyltrimethylammonium bromide. Nat Protoc 1(5):2320–2325 - PubMed - DOI
    1. Arnold AE, Lutzoni F (2007) Diversity and host range of foliar fungal endophytes: are tropical leaves biodiversity hotspots? Ecol 88(3):541–549 - DOI
    1. Bagchi B, Banerjee D (2013) Diversity of fungal endophytes in Bauhinia vahlii (alianas) from different regions of Paschim Medinipur district of West Bengal. IJSET 2:748–756
    1. Beigh SY, Nawchoo IA, Iqbal M (2006) Cultivation and conservation of Aconitum heterophyllum: A critically endangered medicinal herb of the northwest Himalayas. J Herbs Spices Med Plants 11(4):47–56 - DOI

LinkOut - more resources