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. 2023 Aug;239(3):905-919.
doi: 10.1111/nph.18792. Epub 2023 Mar 1.

MOTHER-OF-FT-AND-TFL1 regulates the seed oil and protein content in soybean

Zhandong Cai #  1   2 , Peiqi Xian #  1   3 , Yanbo Cheng #  1   3 , Yiwang Zhong  1   3 , Yuan Yang  1   3 , Qianghua Zhou  1   3 , Tengxiang Lian  1   3 , Qibin Ma  1   3 , Hai Nian  1   3   4 , Liangfa Ge  1   2   4
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Free article

MOTHER-OF-FT-AND-TFL1 regulates the seed oil and protein content in soybean

Zhandong Cai et al. New Phytol. 2023 Aug.
Free article

Abstract

Soybean is a major crop that produces valuable seed oil and protein for global consumption. Seed oil and protein are regulated by complex quantitative trait loci (QTLs) and have undergone intensive selections during the domestication of soybean. It is essential to identify the major genetic components and understand their mechanism behind seed oil and protein in soybean. We report that MOTHER-OF-FT-AND-TFL1 (GmMFT) is the gene of a classical QTL that has been reported to regulate seed oil and protein content in many studies. Mutation of MFT decreased seeds oil content and weight in both Arabidopsis and soybean, whereas increased expression of GmMFT enhanced seeds oil content and weight. Haplotype analysis showed that GmMFT has undergone selection, which resulted in the extended haplotype homozygosity in the cultivated soybean and the enriching of the oil-favorable allele in modern soybean cultivars. This work unraveled the GmMFT-mediated mechanism regulating seed oil and protein content and seed weight, and revealed a previously unknown function of MFT that provides new insights into targeted soybean improvement and breeding.

Keywords: CRISPR/Cas9; fatty acids; natural variation; overexpression; seed.

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References

    1. Abelenda JA, Bergonzi S, Oortwijn M, Sonnewald S, Du M, Visser RGF, Sonnewald U, Bachem CWB. 2019. Source-sink regulation is mediated by interaction of an FT homolog with a SWEET protein in potato. Current Biology 29: 1178-1186.
    1. Akond M, Liu S, Boney M, Kantartzi SK, Meksem K, Bellaloui N, Lightfoot DA, Kassem MA. 2014. Identification of quantitative trait loci (QTL) underlying protein, oil, and five major fatty acids' contents in soybean. American Journal of Plant Sciences 5: 158-167.
    1. Al Amin N, Ahmad N, Wu N, Pu X, Ma T, Du Y, Bo X, Wang N, Sharif R, Wang P. 2019. CRISPR-Cas9 mediated targeted disruption of FAD2-2 microsomal omega-6 desaturase in soybean (Glycine max L.). BMC Biotechnology 19: 9.
    1. Attitalla IH. 2011. Modified CTAB method for high quality genomic DNA extraction from medicinal plants. Pakistan Journal of Biological Sciences 14: 998-999.
    1. Bai MY, Yuan JH, Kuang HQ, Gong PP, Li SN, Zhang ZH, Liu B, Sun JF, Yang MX, Yang L et al. 2020. Generation of a multiplex mutagenesis population via pooled CRISPR-Cas9 in soya bean. Plant Biotechnology Journal 18: 721-731.

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