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. 2022 Dec:10:127-137.
doi: 10.3114/fuse.2022.10.05. Epub 2022 Oct 26.

Globisporangium coniferarum sp. nov., associated with conifers and Quercus spp

F Salmaninezhad  1 , F Aloi  2 , A Pane  2 , R Mostowfizadeh-Ghalamfarsa  1 , S O Cacciola  2
Affiliations

Globisporangium coniferarum sp. nov., associated with conifers and Quercus spp

F Salmaninezhad et al. Fungal Syst Evol. 2022 Dec.

Abstract

During a survey of gardens in Shiraz County, Iran, aimed at identifying oomycetes associated with roots of ornamental trees, a species of Globisporangium with distinctive morphological characters separating it from other known species in this genus was recovered from conifers and occasionally from a Quercus sp. Five isolates of this species were characterised. Phylogenetic analyses of nuclear (ITS and βtub) and mitochondrial (cox1 and cox2) loci using Bayesian inference and maximum likelihood analyses as well as their distinct morphological and cultural characteristics (e.g., abundant production of chlamydospores; globose, ellipsoid to ovoid sporangia; amorphous oogonia with a smooth wall; paragynous to rarely hypogynous antheridia and 1-5 antheridia per oogonium; mostly plerotic oospores) revealed that these isolates belong to a new Globisporangium species grouping in the phylogenetic clade G of Pythium sensu lato. This paper formally describes Globisporangium coniferarum sp. nov. as a new species and compares it with other phylogenetically related and already known Globisporangium species, including G. nagaii, G. violae, G. paddicum, G. okanoganense, G. iwayamae and G. canariense. Citation: Salmaninezhad F, Aloi F, Pane A, Mostowfizadeh-Ghalamfarsa R, Cacciola SO (2022). Globisporangium coniferarum sp. nov., associated with conifers and Quercus spp. Fungal Systematics and Evolution 10: 127-137. doi: 10.3114/fuse.2022.10.05.

Keywords: Conifers; Globisporangium; Pythiaceae; new taxon; oomycete; phylogenetic analyses.

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Conflict of interest statement

Conflict of interest: The authors declare that there is no conflict of interest.

Figures

Fig. 1.
Fig. 1.
Phylogenetic relationships of Globisporangium isolates from conifers and Quercus sp. (Shiraz County, Iran) among 20 Globisporangium species based on Bayesian analysis of multigene genealogies of nuclear (ITS and βtub) and mitochondrial (cox1 and cox2) sequences. Numbers on branches represent posterior probability based on Bayesian analysis and the bootstrap support based on maximum likelihood analysis, respectively.
Fig. 2.
Fig. 2.
Colony morphology of Globisporangium coniferarum CBS 148568 (after 48 h) on various media at 25 °C; top (from left to right): carrot agar, malt extract agar and potato-dextrose agar; bottom (from left to right): cornmeal agar and hempseed agar.
Fig. 3.
Fig. 3.
Morphology of Globisporangium coniferarum. A, B. Sporangia: A. Terminal ovoid sporangium with pedicel (arrow); B. Globose sporangia. C–K. Sexual structures: C. Formation of intercalary aplerotic oogonium with paragynous diclinous antheridium (arrow); D. Plerotic ellipsoid oospore; E. Asymmetrical plerotic oospore with diclinous clavate antheridium (arrow); F. Plerotic terminal oospore; G. Nearly aplerotic globose oospore; H. Ovoid oospore with intercalary, paragynous and diclinous antheridium (arrow); I. Intercalary plerotic oospore with hypogynous antheridia (arrows); J. Globose oospore; K. Intercalary nearly aplerotic oospore with paragynous, monoclinous antheridium originated near oogonium (arrow). L–N. Chlamydospores: L. Terminal globose chlamydospore; M. Intercalary globose chlamydospore; N. Intercalary ovoid chlamydospore. O. Amorphous to ovoid chlamydospore. Scale bar = 10 μm.
Fig. 4.
Fig. 4.
Average radial growth rate of Globisporangium coniferarum isolates on potato-dextrose agar at different temperatures.
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