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. 2023 Jan 20:14:1087121.
doi: 10.3389/fpls.2023.1087121. eCollection 2023.

Transcriptome analysis provides StMYBA1 gene that regulates potato anthocyanin biosynthesis by activating structural genes

Xijuan Zhao  1   2 , Huiling Zhang  3 , Tengfei Liu  2 , Yanan Zhao  3 , Xinxi Hu  1 , Shengxuan Liu  2 , Yuan Lin  1 , Botao Song  2 , Changzheng He  1
Affiliations

Transcriptome analysis provides StMYBA1 gene that regulates potato anthocyanin biosynthesis by activating structural genes

Xijuan Zhao et al. Front Plant Sci. .

Abstract

Anthocyanin biosynthesis is affected by light, temperature, and other environmental factors. The regulation mode of light on anthocyanin synthesis in apple, pear, tomato and other species has been reported, while not clear in potato. In this study, potato RM-210 tubers whose peel will turn purple gradually after exposure to light were selected. Transcriptome analysis was performed on RM-210 tubers during anthocyanin accumulation. The expression of StMYBA1 gene continued to increase during the anthocyanin accumulation in RM-210 tubers. Moreover, co-expression cluster analysis of differentially expressed genes showed that the expression patterns of StMYBA1 gene were highly correlated with structural genes CHS and CHI. The promoter activity of StMYBA1 was significantly higher in light conditions, and StMYBA1 could activate the promoter activity of structural genes StCHS, StCHI, and StF3H. Further gene function analysis found that overexpression of StMYBA1 gene could promote anthocyanin accumulation and structural gene expression in potato leaves. These results demonstrated that StMYBA1 gene promoted potato anthocyanin biosynthesis by activating the expression of structural genes under light conditions. These findings provide a theoretical basis and genetic resources for the regulatory mechanism of potato anthocyanin synthesis.

Keywords: StMYBA1; anthocyanin biosynthesis; light-induced; potato; transcriptional activation.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Tuber phenotype and anthocyanin content of RM-210 tubers. (A) The phenotype of RM-210 tubers during light treatment. (B) The phenotype of the RM-210 tubers exposed to light 48 h. (C) Delphinidin content of RM-210 tubers.
Figure 2
Figure 2
Overview of transcriptome analysis. (A) Correlation analysis of RNA-seq data of different treatments. (B) The number of differentially expressed genes (DEGs) in each comparison. (C) Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis of DEGs in each comparison.
Figure 3
Figure 3
Cluster analysis of gene expression patterns in RM-210 tubers at different time points of light treatment. The Y-axis represents the deviation of the gene expression. The X-axis represents the sampling time points.
Figure 4
Figure 4
Co-expression analysis of structural genes and transcription factors in DEGs. (A) Anthocyanin biosynthesis pathway. (B) Co-expression cluster analysis of structural genes and transcription factors. The red boxes mark transcription factors that have a high correlation with the expression of key structural genes.
Figure 5
Figure 5
Light Response analysis of StMYBA1 Gene and its regulation on structural genes. (A) Distribution of main core elements of StMYBA1 and structural gene promoters. (B) Activity analysis of StMYBA1 promoter under light and dark conditions. (C) Regulation of StMYBA1 on structural genes related to anthocyanin biosynthesis. Significant differences (LSD) between means of the dark and light treatments, and pSAK-277 and StMYBA1 are indicated by asterisks (** p < 0.01).
Figure 6
Figure 6
Regulation of StMYBA1 gene on anthocyanin biosynthesis in potato. (A) Representative leaves images of StMYBA1-overexpressed transgenic lines (OE-7, OE-8, and OE-10) and the wild type (AC142). Bars=2 cm. (B) The anthocyanin content in the StMYBA1 transgenic leaves. (C–K) The relative expression of StMYBA1 and structural genes in the transgenic leaves. Significant differences (LSD) between means of the transgenic lines and wild type are indicated by asterisks (* p < 0.05, ** p < 0.01).
Figure 7
Figure 7
The regulatory model of StMYBA1 gene on potato anthocyanin biosynthesis.
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