doi: 10.1016/0003-2697(87)90370-8.
Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry
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- PMID: 3674373
- DOI: 10.1016/0003-2697(87)90370-8
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Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry
Anal Biochem.
1987 Jul.
Abstract
Europium has been used as a label in immunoassays as it can be measured with high sensitivity by means of time-resolved fluorometry. Here we have used streptavidin labeled with europium chelates in the detection of adenovirus type 2 DNA bound to microtiter wells after hybridization with a biotinylated probe. The method gave quantitative results and a sensitivity of about 10 pg of the specific DNA.
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