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. 1987 Jul;164(1):78-83.
doi: 10.1016/0003-2697(87)90370-8.

Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry

P Dahlén  1
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Detection of biotinylated DNA probes by using Eu-labeled streptavidin and time-resolved fluorometry

P Dahlén. Anal Biochem. 1987 Jul.

Abstract

Europium has been used as a label in immunoassays as it can be measured with high sensitivity by means of time-resolved fluorometry. Here we have used streptavidin labeled with europium chelates in the detection of adenovirus type 2 DNA bound to microtiter wells after hybridization with a biotinylated probe. The method gave quantitative results and a sensitivity of about 10 pg of the specific DNA.

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