Waning Immunity Against XBB.1.5 Following Bivalent mRNA Boosters

Abstract

The SARS-CoV-2 Omicron variant has continued to evolve. XBB is a recombinant between two BA.2 sublineages, XBB.1 includes the G252V mutation, and XBB.1.5 includes the G252V and F486P mutations. XBB.1.5 has rapidly increased in frequency and has become the dominant virus in New England. The bivalent mRNA vaccine boosters have been shown to increase neutralizing antibody (NAb) titers to multiple variants, but the durability of these responses remains to be determined. We assessed humoral and cellular immune responses in 30 participants who received the bivalent mRNA boosters and performed assays at baseline prior to boosting, at week 3 after boosting, and at month 3 after boosting. Our data demonstrate that XBB.1.5 substantially escapes NAb responses but not T cell responses after bivalent mRNA boosting. NAb titers to XBB.1 and XBB.1.5 were similar, suggesting that the F486P mutation confers greater transmissibility but not increased immune escape. By month 3, NAb titers to XBB.1 and XBB.1.5 declined essentially to baseline levels prior to boosting, while NAb titers to other variants declined less strikingly.

Figure 1.. Humoral and cellular immune responses to SARS-CoV-2 Omicron variants.

A. Spike sequences for BA.2, BA.5, BQ.1.1, XBB parental sequences BJ.1 (BA.2.10.1) and a sublineage of BA.2.75 (BM.1.1.1), XBB.1, and XBB.1.5 are depicted. Mutations compared with the ancestral WA1/2020 Spike are shown in black, and additional mutations relative to BA.2 are highlighted in colors corresponding to individual variants. For the XBB.1 and XBB.1.5 sequences, black reflects mutations from BA.2, red reflects additional mutations from BJ.1, blue reflects additional mutations from BA.2.75, and green reflects new mutations. NTD, N-terminal domain; RBD, receptor binding domain. B. Neutralizing antibody (NAb) titers against the WA1/2020, BA.2, BA.5, BQ.1.1, XBB.1, and XBB.1.5 variants by luciferase-based pseudovirus neutralization assays at baseline prior to boosting, at week 3 after boosting, and at month 3 after boosting in nucleocapsid seronegative participants. C. Spike-specific, IFN-γ CD4+ and CD8+ T cell responses to pooled WA1/2020, BQ.1.1, and XBB.1.5 peptides by intracellular cytokine staining assays at baseline prior to boosting and at month 3 after boosting. Dotted lines reflect limits of quantitation. Medians (red bars) are depicted and shown numerically.

Figure 1.. Humoral and cellular immune responses to SARS-CoV-2 Omicron variants.

A. Spike sequences for BA.2, BA.5, BQ.1.1, XBB parental sequences BJ.1 (BA.2.10.1) and a sublineage of BA.2.75 (BM.1.1.1), XBB.1, and XBB.1.5 are depicted. Mutations compared with the ancestral WA1/2020 Spike are shown in black, and additional mutations relative to BA.2 are highlighted in colors corresponding to individual variants. For the XBB.1 and XBB.1.5 sequences, black reflects mutations from BA.2, red reflects additional mutations from BJ.1, blue reflects additional mutations from BA.2.75, and green reflects new mutations. NTD, N-terminal domain; RBD, receptor binding domain. B. Neutralizing antibody (NAb) titers against the WA1/2020, BA.2, BA.5, BQ.1.1, XBB.1, and XBB.1.5 variants by luciferase-based pseudovirus neutralization assays at baseline prior to boosting, at week 3 after boosting, and at month 3 after boosting in nucleocapsid seronegative participants. C. Spike-specific, IFN-γ CD4+ and CD8+ T cell responses to pooled WA1/2020, BQ.1.1, and XBB.1.5 peptides by intracellular cytokine staining assays at baseline prior to boosting and at month 3 after boosting. Dotted lines reflect limits of quantitation. Medians (red bars) are depicted and shown numerically.

Figure 1.. Humoral and cellular immune responses to SARS-CoV-2 Omicron variants.

A. Spike sequences for BA.2, BA.5, BQ.1.1, XBB parental sequences BJ.1 (BA.2.10.1) and a sublineage of BA.2.75 (BM.1.1.1), XBB.1, and XBB.1.5 are depicted. Mutations compared with the ancestral WA1/2020 Spike are shown in black, and additional mutations relative to BA.2 are highlighted in colors corresponding to individual variants. For the XBB.1 and XBB.1.5 sequences, black reflects mutations from BA.2, red reflects additional mutations from BJ.1, blue reflects additional mutations from BA.2.75, and green reflects new mutations. NTD, N-terminal domain; RBD, receptor binding domain. B. Neutralizing antibody (NAb) titers against the WA1/2020, BA.2, BA.5, BQ.1.1, XBB.1, and XBB.1.5 variants by luciferase-based pseudovirus neutralization assays at baseline prior to boosting, at week 3 after boosting, and at month 3 after boosting in nucleocapsid seronegative participants. C. Spike-specific, IFN-γ CD4+ and CD8+ T cell responses to pooled WA1/2020, BQ.1.1, and XBB.1.5 peptides by intracellular cytokine staining assays at baseline prior to boosting and at month 3 after boosting. Dotted lines reflect limits of quantitation. Medians (red bars) are depicted and shown numerically.