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. 2022 Dec;72(12):005608.
doi: 10.1099/ijsem.0.005608.

Bdellovibrio reynosensis sp. nov., from a Mexico soil sample

Yewande Olajumoke Ajao  1 , Isabel Cristina Rodríguez-Luna  1 , Temidayo Oluyomi Elufisan  2 , Alejandro Sánchez-Varela  1 , Diana Verónica Cortés-Espinosa  3 , Andrew Camilli  4 , Xianwu Guo  1
Affiliations

Bdellovibrio reynosensis sp. nov., from a Mexico soil sample

Yewande Olajumoke Ajao et al. Int J Syst Evol Microbiol. 2022 Dec.

Abstract

A novel predatory bacterium, strain LBG001T, has been isolated from Reynosa, Mexico. The 16S rRNA shares approximately 97 % sequence identity with many reported strains in the genus Bdellovibrio including the type strain Bdellovibrio bacteriovorus HD100T. Phylogenetic trees based on the 16S rRNA gene and on 30 concatenated housekeeping genes or core genes showed that LBG001T is on a separate branch from the B. bacteriovorus group. LBG0001T has a genome size of 3 582 323 bp with a G+C content of 43.1 mol %. The average nucleotide identity, average amino acid identity and digital DNA-DNA hybridization values with other members of the genus Bdellovibrio (<79, <72 and <17 %, respectively) qualifies the strain to represent a new species in the genus. Strain LBG001T formed visible plaques on all 10 tested Gram-negative bacterial species. The phenotypic characteristics, phylogenetic analysis and genomic taxonomic studies support the classification of the strain as representing a new species for which the name Bdellovibrio reynosensis sp. nov. is proposed. The type strain is LBG001T(=ATCC TSD-288T =CM-CNRG 0932T).

Keywords: Bdellovibrio reynosensis; genome comparison; genome sequence; new species; predatory bacteria.

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Conflict of interest statement

The authors declare that there are no conflicts of interest.

Figures

Fig. 1.
Fig. 1.
Neighbour-joining tree reconstructed using 16S rRNA gene sequences.
Fig. 2.
Fig. 2.
Concatenated phylogenetic tree of 30 housekeeping genes showing the relatedness of Bdellovibrio reynosensis sp. nov. to the type strain B. bacteriovorus HD100T.
Fig. 3.
Fig. 3.
The concatenated phylogenetic tree of core genes shared by 11 complete whole-genome sequenced Bdellovibrio . Concatenated core genes were recovered from the BPGA analysis. The tree was reconstructed on mega X using default parameters at 1000 bootstraps.
Fig. 4.
Fig. 4.
Bdellovibrio predation of Klebsiella pneumoniae . B. bacteriovorus (a, c) and B. reynosensis sp. nov. (b, d) were fluorescently labelled, added to unlabeled K. pneumoniae resuspended in HEPES buffer, and incubated at 30 °C with aeration. After 10 and 80 min, cells were placed on agarose pads on slides and examined by bright field and fluorescence microscopy. Results were equivalent for both Bdellovibrio species. At 10 min, uninfected large, rod-shaped prey cells and small, extracellular but mostly attached fluorescent predator cells (filled arrows) are visible. At 80 min, prey cells have rounded into bdelloplasts with intracellular fluorescent predator (open arrows). Inset boxes in the upper right of some panels show enlarged images of example attached predator (a, b) and bdelloplasts (c, d).
Fig. 5.
Fig. 5.
Bdellovibrio reynosensis LBG001T growing as plaques on a lawn plate of Klebsiella oxytocoa.
See this image and copyright information in PMC

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