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. 2023 Feb 9;21(1):12.
doi: 10.1186/s43141-023-00471-3.

Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

Sahar Saleh Mohamed  1 , Mohamed E El Awady  1 , Sayeda Abdelrazek Abdelhamid  2 , Ahmed Abdelghani Hamed  3 , Abeer A A Salama  4 , Manal S Selim  1
Affiliations

Study of exopolysaccharide produced by Streptomyces rochie strain OF1 and its effect as ameliorative on osteoarthritis in rats via inhibiting TNF-α/COX2 pathway

Sahar Saleh Mohamed et al. J Genet Eng Biotechnol. .

Abstract

Background: Carbohydrates are known as the main natural products of life activities.

Results: Streptomyces rochie strain OF1 isolated from a mangrove tree produced exopolysaccharide S5 (EPSS5) (14.2 gl-1) containing uronic acid 21.98% sulfate content of 11.65 mg/ml, and a viscosity of 1.35 mm2/s. while total hexose amine content was 24.72%. The high performance liquid chromatography (HPLC) analysis of mono sugars revealed that EPS was composed of manouronic acid, glucuronic acid, xylose, and fructose at a molar ratio of 1.0:0.5:1.0:2.0, respectively. It showed that the whole antioxidant activity was 92.06%. It showed antibacterial activity against Staphylococcus aureus, and E. coli, MRSA and Klebsiella pneumoniae. But, EPSS5 displayed low antifungal activity against Candida albicans. While no antifungal activity has been detected against Aspergillus niger. EPSS5 has antibiofilm action that is noticeable toward S. aureus with an inhibition ratio of biofilm up to 50%. Effect of EPS on serum levels of TNF-α and COX2 by 2 fold and 1.9 fold of EPS reduced serum levels of Tumor necrosis factor-α (TNF-α) by 38%, 12%, 49%, and Cyclooxygenase-2 (COX2) by 61%, 34%, and 62%, respectively. By affected of EPSS5 on arthritis in rats stimulated by carrageenan.

Conclusions: Administration of EPS ameliorated carrageen-induced elevation in inflammatory mediators; TNF-α/COX and suppressed the expressions of metalloproteinase 9 (MMP9) by 68%, 86%, and 75% correspondingly in comparison to the group of carrageenans. Then again, therapy involving a high dose only reduced MMP9 level by 57%, compared to free drug suggesting that EPSS5 is a good inhibitor of the MMP9, as it brought MMP9 back to normal levels via the signaling pathway.

Keywords: Antibiofilm; Antimicrobial; Exopolysaccharide; Inflammatory biomarkers; Streptomyces rochie.

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Conflict of interest statement

The authors declare that they have no competing interests.

Figures

Fig. 1
Fig. 1
Electron micrograph of spores of Streptomyces rochie strain OF1 (X 14 000)
Fig. 2
Fig. 2
Phylogenetic tree of Streptomyces rochie strain OF1, a local isolate in a using its incomplete 16S rRNA sequencing in comparison to nearby sequences found in GenBank databases
Fig. 3
Fig. 3
FTIR for Streptomyces rochie strain OF1 EPS
Fig. 4
Fig. 4
EPSS5 free radical scavenging capability against DPPH
Fig. 5
Fig. 5
Antimicrobial activity of EPSS5
Fig. 6
Fig. 6
Biofilm inhibitory activity of EPSS5
Fig. 7
Fig. 7
Effect of EPSS5 on serum levels of TNF-α and COX2. The data was presented as mean ± SE (n = 8). Fisher‘s LSD test for multiple comparisons was used after one-way ANOVA in the statistical analysis. At P ˂ 0.05, various letters show significant differences
Fig. 8
Fig. 8
Effect EBS on MMP9 serum level. The data were presented as mean ± SE (n = 8). Fisher's LSD test for multiple comparisons was used after one-way ANOVA in the statistical analysis. At P ˂ 0.05, various letters are statistically distinct
See this image and copyright information in PMC

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