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. 2023 Feb 5;8(2):215-219.
doi: 10.1080/23802359.2023.2171246. eCollection 2023.

Complete mitochondrial genome of a livebearing freshwater fish (Cyprinodontiformes: Poeciliidae): Poecilia parae

Kayla M Fast  1 , Alex W Rakestraw  1 , Michael W Sandel  2
Affiliations

Complete mitochondrial genome of a livebearing freshwater fish (Cyprinodontiformes: Poeciliidae): Poecilia parae

Kayla M Fast et al. Mitochondrial DNA B Resour. .

Abstract

Members of the fish family Poeciliidae (livebearing 'tooth-carps') have historically been used as models in medical research, behavior ecology, and biological control. This group of primarily freshwater fishes is highly tolerant to environmental factors such as salinity and warm temperatures and includes some invasive species. Here, we present the mitochondrial genome of Poecilia parae. A representative of this species was obtained from Suriname. The complete mitochondrial genome was sequenced using Oxford Nanopore technology and is 16,559 bp long. The genome contains 13 protein-coding genes, two ribosomal RNAs (rRNAs), 22 transfer RNAs (tRNAs), and one control region (D-loop). Phylogenetic analysis yielded topologies similar to those previously published. The data generated here will be useful in future studies of comparative biology and those utilizing environmental DNA (eDNA).

Keywords: Oxford Nanopore; Poecilia parae; Poeciliidae; mitochondrial genome.

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Conflict of interest statement

The authors have no conflicts of interest to declare.

Figures

Figure 1.
Figure 1.
Representative photograph of Poecilia parae (blue melanzona morph).
Figure 2.
Figure 2.
Mitochondrial genome map of Poecilia parae. The innermost circle of the image represents %GC per every 5 bp of the mitogenome; longer lines indicate higher %GC.
Figure 3.
Figure 3.
Maximum-likelihood phylogeny reconstructed using mitochondrial protein-coding sequences under the GTR + G+I model and 1000 bootstrap replicates. The following sequences were used: AP005982 (Miya et al. 2003), KT594624 (Zhang et al. 2016), MW934558 (Eastis et al. 2021), ON797008, FJ226476 (Bai et al. 2009), FJ234985 (Bai et al. 2009), CM021098 (van Kruistum et al. 2020), KJ013505 (Kong et al. 2016), KJ460033 Künstner et al. (2016), OP326603 (this study), KT166983 (Dang et al. 2016), KT175513, KT307617 (Sung et al. 2016), KT715811, MK263672, KT175514, KT175512, KT175511, LC026151 (Jiang et al. 2016), KX229692 (Jeon et al. 2016), MZ681841, MK860197 (Mateos et al. 2019), KP013108, MK860198 (Mateos et al. 2019), OL825609, OL457416, AP004422 (Miya et al. 2003), KP013085, KP013115, and AP006777 (Setiamarga et al. 2008). Numbers on nodes are bootstrap support values. The sequence generated in this study is written in bold font and marked with an asterisk.
See this image and copyright information in PMC

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