Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2022 Dec 7:1134:52-73.
doi: 10.3897/zookeys.1134.89267. eCollection 2022.

A new species of Andricus Hartig, 1840 (Hymenoptera, Cynipidae) from China, with references to DNA taxonomy and Wolbachia infection

Yin Pang  1 , Cheng-Yuan Su  1 , Jun-Qiao Zhu  1 , Xiao-Hui Yang  2 , Jia-Lian Zhong  1 , Dao-Hong Zhu  1 , Zhiwei Liu  3
Affiliations

A new species of Andricus Hartig, 1840 (Hymenoptera, Cynipidae) from China, with references to DNA taxonomy and Wolbachia infection

Yin Pang et al. Zookeys. .

Abstract

In the present paper, a new species of cynipid gall wasp, Andricuselodeoides Liu & Pang, is described from several provinces in southern China. The new species is closely related to the recently redescribed A.mairei (Kieffer, 1906). In addition to differences in adult and gall morphology, the new species is also readily separated by COI sequences, with a 6.2-8.9% genetic distance between populations of the new species and those of A.mairei. A contrasting difference in sex ratios was also observed between the two species, with A.elodeoides extremely female-biased (95.5-97.8% female) while A.mairei male-biased to more balanced (5.4-43.5% female). PCR screening for Wolbachia infection further revealed contrasting infection rates between populations of A.elodeoides and A.mairei: the Wolbachia infection rate was 0% in A.elodeoides and 100% in A.mairei. Cytoplasmic incompatibility induced by Wolbachia is proposed as a potential mechanism of speciation of the sympatric A.elodeoides and A.mairei.

Keywords: Andricuselodeoides; Quercusserrata; gall wasp; phylogeny; taxonomy.

PubMed Disclaimer

Figures

Figures 1–6.
Figures 1–6.
Andricuselodeoides sp. nov., female 1 head in anterior view 2 head in posterior view 3 head in dorsal view 4 metasoma in lateral view 5 mesosoma in dorsal view 6 mesosoma in lateral view.
Figures 7–13.
Figures 7–13.
Andricuselodeoides sp. nov. 7 propodeum of female in dorsal view 8 general habitus of male 9 female antenna 10 male antenna 11 general habitus of female 12 female forewing 13 the claw of hind leg of female.
Figures 14, 15.
Figures 14, 15.
Galls on Quercusserrata14Andricuselodeoides sp. nov. 15Andricusmairei.
Figure 16.
Figure 16.
Bayesian phylogenetic tree of A.elodeoides sp. nov. and other Andricus species based on COI and 28S sequences. Bold font refers to the sequence obtained in this study, and others are downloaded from NCBI. The photograph on the right refers to the gall of adult emergence. The letters in parentheses indicate the sampled populations shown in Table 1. The length of the branches is drawn to scale of genetic distance and the number over branches is posterior probability. Melikaiellabicolor and Dryocosmusliui were used as the outgroup.
Figure 17.
Figure 17.
Bayesian phylogenetic tree for A.elodeoides sp. nov. and A.mairei of different geographic populations using COI sequences. Bold font refers to the sequence obtained in this study, and the others are from Pujade-Villar et al. (2020). The letters in parentheses indicate the sampled populations shown in Table 1. The length of the branches is drawn to scale and show the genetic distances, and the number over branches is posterior probability.
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Abe Y, Miura K. (2002) Doses Wolbachia induce unisexuality in oak gall wasps? (Hymenoptera: Cynipidae). Annals of the Entomological Society of America 95(5): 583–586. 10.1603/0013-8746(2002)095[0583:DWIUIO]2.0.CO;2 - DOI
    1. Baldo L, Dunning Hotopp JC, Bordenstein SR, Biber S, Jollie K, Hayashi C, Tettelin H, Maiden M, Werren JH. (2006) A multilocus sequence typing system for the endosymbiont Wolbachia. Molecular Biology and Evolution 23(2): 437–449. 10.1093/molbev/msj049 - DOI - PubMed
    1. Bordenstein S, O’Hara FP, Werren JH. (2001) Wolbachia-induced incompatibility precedes other hybrid incompatibilities in Nasonia. Nature 409(6821): 707–710. 10.1038/35055543 - DOI - PubMed
    1. Cook TD, Campbell DT, Shadish W. (2002) Experimental and quasi-experimental designs for generalized causal inference. Houghton Mifflin, Boston, 1–81. 10.1198/jasa.2005.s22 - DOI
    1. Cuesta-Porta V, Melika G, Nichols JA, Stone G, Pujade-Villar J. (2022) Reestablishment of the Nearctic oak cynipid gall wasp genus Druon Kinsey, 1937 (Hymenoptera: Cynipidae: Cynipini), with description of five new species. Zootaxa 5132: 001–092. 10.11646/zootaxa.5132.1.1 - DOI - PubMed

LinkOut - more resources