Immunoradiometric assay of endotoxin in serum
- PMID: 367636
Item in Clipboard
Immunoradiometric assay of endotoxin in serum
Clin Chem.
1979 Jan.
Abstract
We describe an immunoradiometric assay for the specific detection and quantitation of endotoxin from Escherichia coli 026, with use of 125I-labeled antibody. The sensitivty of this assay is 1 microgram/L.E. coli 026 endotoxin was detected in serum of rats for as long as 24 h after intraperitoneal injection of 0.1 mg of endotoxin per 100 g body weight. The assay is specific for the O-polysaccharide portion of this endotoxin, as confirmed by results after protein digestion and oxidation of the endotoxin, as well as by failure to detect endotoxin in serum after similar injections of E. coli 0127 and Salmonella typhosa 0901 endotoxins.
Similar articles
-
Immunoradiometric assay of lipid A: a test for detecting and quantitating endotoxins of various origins.J Immunol Methods. 1982 Nov 26;55(1):63-72. doi: 10.1016/0022-1759(82)90077-1. J Immunol Methods. 1982. PMID: 6759581
-
Measurement of endotoxin. I. Fundamental studies of radioimmunoassay of endotoxin.Acta Med Okayama. 1976 Aug;30(4):245-55. Acta Med Okayama. 1976. PMID: 137652
-
J5 prevents endotoxin shock in suckling rats.Res Commun Chem Pathol Pharmacol. 1993 Jun;80(3):367-70. Res Commun Chem Pathol Pharmacol. 1993. PMID: 8351416
-
Effects of urethane, ambient temperature and injection route on rat body temperature and metabolism due to endotoxins.J Physiol. 1988 Nov;405:547-62. doi: 10.1113/jphysiol.1988.sp017347. J Physiol. 1988. PMID: 2475610 Free PMC article.
-
Absence of endotoxin fever but not prostaglandin E2 fever in the Brattleboro rat.Am J Physiol. 1982 Jan;242(1):R116-20. doi: 10.1152/ajpregu.1982.242.1.R116. Am J Physiol. 1982. PMID: 7036757
Cited by
-
Radioimmunoassay for Gram-negative bacterial lipopolysaccharide O antigens: influence of antigen solubility.Infect Immun. 1979 Oct;26(1):42-8. doi: 10.1128/iai.26.1.42-48.1979. Infect Immun. 1979. PMID: 387612 Free PMC article.
-
Endotoxemia: methods of detection and clinical correlates.Clin Microbiol Rev. 1995 Apr;8(2):268-92. doi: 10.1128/CMR.8.2.268. Clin Microbiol Rev. 1995. PMID: 7621402 Free PMC article. Review.
-
Measurement of endotoxaemia by the Limulus test.Intensive Care Med. 1984;10(1):1-2. doi: 10.1007/BF00258060. Intensive Care Med. 1984. PMID: 6699263 No abstract available.
-
The contribution of gut-derived endotoxins to liver injury.Yale J Biol Med. 1979 Jan-Feb;52(1):127-33. Yale J Biol Med. 1979. PMID: 377823 Free PMC article.