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. 2023 Jan 26;13(3):412.
doi: 10.3390/ani13030412.

Effects of Dietary Glycine Supplementation on Growth Performance, Immunological, and Erythrocyte Antioxidant Parameters in Common Carp, Cyprinus carpio

Marzieh Abbasi  1 , Ali Taheri Mirghaed  2 , Seyyed Morteza Hoseini  3 , Hamid Rajabiesterabadi  4 , Seyed Hossein Hoseinifar  5 , Hien Van Doan  5   6
Affiliations

Effects of Dietary Glycine Supplementation on Growth Performance, Immunological, and Erythrocyte Antioxidant Parameters in Common Carp, Cyprinus carpio

Marzieh Abbasi et al. Animals (Basel). .

Abstract

The effects of dietary glycine supplementation, 0 (control), 5 (5 GL), and 10 (10 GL) g/kg, have been investigated on growth performance, hematological parameters, erythrocyte antioxidant capacity, humoral and mucosal immunity in common carp, Cyprinus carpio. After eight weeks feeding, the 5 GL treatment exhibited significant improvement in growth performance and feed efficacy, compared to the control treatment. Red blood cell (RBC) and white blood cell (WBC) counts, hemoglobin, hematocrit, neutrophil and monocyte counts/percentages, RBC reduced glutathione (GSH) content, and skin mucosal alkaline phosphatase, peroxidase, protease, and lysozyme activities were similar in the glycine-treated fish and significantly higher than the control treatment. Blood lymphocyte percentage decreased in the glycine-treated fish, but lymphocyte count increased, compared to the control fish. RBC glutathione reductase activities in the glycine-treated fish were similar and significantly lower than the control treatment. The highest plasma lysozyme and alternative complement activities were observed in GL treatment. The glycine-treated fish, particularly 5 GL, exhibited significant improvement in RBC osmotic fragility resistance. Dietary glycine had no significant effects on RBC glutathione peroxidase activity, plasma immunoglobulin, eosinophil percentage/count, and hematological indices. In conclusion, most of the benefits of dietary glycine supplementation may be mediated by increased glutathione synthesis and antioxidant power.

Keywords: amino acid; blood cells; glutathione; nutrition; skin mucus.

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Conflict of interest statement

The authors declare no conflict of interest.

References

    1. Mai K., Xue M., He G., Xie S.Q., Kaushik S.J. Chapter 4—Protein and amino acids. In: Hardy R.W., Kaushik S.J., editors. Fish Nutrition. 4th ed. Academic Press; Oxford, UK: 2022. pp. 181–302.
    1. Li P., Mai K.S., Trushenski J., Wu G.Y. New developments in fish amino acid nutrition: Towards functional and environmentally oriented aquafeeds. Amino Acids. 2009;37:43–53. doi: 10.1007/s00726-008-0171-1. - DOI - PubMed
    1. Hoseini S.M., Reverter M. Health-promoting effects of amino acids in fish. In: Gupta S.K., Giri S.S., editors. Biotechnological Advances in Aquaculture Health Management. Springer Singapore; Singapore: 2021. pp. 493–533.
    1. Li P., Wu G. Roles of dietary glycine, proline, and hydroxyproline in collagen synthesis and animal growth. Amino Acids. 2018;50:29–38. doi: 10.1007/s00726-017-2490-6. - DOI - PubMed
    1. Pérez-Torres I., María Zuniga-Munoz A., Guarner-Lans V. Beneficial effects of the amino acid glycine. Mini. Rev. Med. Chem. 2017;17:15–32. doi: 10.2174/1389557516666160609081602. - DOI - PubMed

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