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. 2023 Feb 3;13(3):538.
doi: 10.3390/ani13030538.

Detection of the Endangered Siamese Bat Catfish (Oreoglanis siamensis Smith, 1933) in Doi Inthanon National Park Using Environmental DNA

Thanatrinan Rodpai  1 , Chatmongkon Suwannapoom  2 , Maslin Osathanunkul  1   3
Affiliations

Detection of the Endangered Siamese Bat Catfish (Oreoglanis siamensis Smith, 1933) in Doi Inthanon National Park Using Environmental DNA

Thanatrinan Rodpai et al. Animals (Basel). .

Abstract

Siamese bat catfish (Oreoglanis siamensis Smith, 1993) has been listed as an endangered species, and its abundance has been severely declining due to habitat degradation and overfishing. To establish an appropriate management strategy, it is crucial to gain information about the distribution of this endangered species. As O. siamensis live under rocks in streams, detecting their presence is difficult. Recently, environmental DNA (eDNA)-based detection has been demonstrated to be a valid tool for monitoring rare species, such as O. siamensis. Therefore, this study developed an eDNA assay targeting a 160 bp fragment of the COI region to detect the presence of this species in its natural habitat. An amount of 300 mL of water samples (0.7 μm filtered) were collected from 15 sites in the Mae Klang sub-basin, where this fish species was visually detected at two locations. O. siamensis eDNA was detected at 12 of the 15 sites sampled with varying concentrations (0.71-20.27 copies/mL), including at the sites where this species was visually detected previously. The developed O. siamensis eDNA assay was shown to be effective for detecting the presence of this endangered species in the Klang Phat and Klang Rivers within the Doi Inthanon National Park.

Keywords: Thai national park; conservation plan; endemic and endangered fish; environmental DNA; nondestructive methods.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Locations of the sampling sites with indication of previous visual observation. (A) Map of Thailand, (B) a general map of the Doi Inthanon National Park with details of the Klang Phat River and Klang River, (C) location of the sampling sites (N = 15) and visual detection of the target species in this study (N = 2) across the Klang Phat River and Klang River in the Doi Inthanon National Park, Chiang Mai, Thailand.
Figure 2
Figure 2
Turbid water at sampling sites. (A) KP4 from the Klang Phat River and (B) K11 from the Klang River.
See this image and copyright information in PMC

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