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. 2023 Jan 31;13(3):523.
doi: 10.3390/diagnostics13030523.

Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media

Alessandra Natale  1 , Saoussen Oueslati  2   3 , Alice Rochard  1 , Sien Ombelet  4 , Daniel Lopez-Baez  5 , Liselotte Hardy  4 , Jane Cunningham  6 , Céline Franquesa  1 , Olivier Vandenberg  7   8   9 ; Mini-Lab Scientific Committee; Jean-Baptiste Ronat  1   2   3 , Thierry Naas  2   3
Affiliations

Evaluation of InTray Cassettes Directly from Blood Cultures for the Diagnosis of Sepsis in Clinical Bacteriology Laboratories as an Alternative to Classic Culture Media

Alessandra Natale et al. Diagnostics (Basel). .

Abstract

Culture media is fundamental in clinical bacteriology for the detection and isolation of bacterial pathogens. However, in-house media preparation could be challenging in low-resource settings. InTray® cassettes (Biomed Diagnostics) could be a valid alternative as they are compact, ready-to-use media preparations. In this study, we evaluate the use of two InTray media as a subculture alternative for the diagnosis of bloodstream infections: the InTray® Müller-Hinton (MH) chocolate and the InTray® Colorex™ Screen. The InTray MH chocolate was evaluated in 2 steps: firstly, using simulated positive blood cultures (reference evaluation study), and secondly, using positive blood cultures from a routine clinical laboratory (clinical evaluation study). The Colorex Screen was tested using simulated poly-microbial blood cultures. The sensitivity and specificity of the InTray MH chocolate were respectively 99.2% and 90% in the reference evaluation study and 97.1% and 88.2% in the clinical evaluation study. The time to detection (TTD) was ≤20 h in most positive blood cultures (99.8% and 97% in the two studies, respectively). The InTray® MH Chocolate agar showed good performance when used directly from clinical blood cultures for single bacterial infections. However, mixed flora is more challenging to interpret on this media than on Colorex™ Screen, even for an experienced microbiologist.

Keywords: Colorex™ screen; InTray cassettes; InTray® Müller-Hinton (MH) chocolate; LMICs; blood cultures; bloodstream infection; clinical bacteriology in low resource settings; diagnosis of sepsis; polymicrobial infections; subcultures.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure A1
Figure A1
Inoculation technique for InTray cassettes: (a) expand the drop by streaking on half the surface (1, first quarter); (b) streak on a new quarter (2, second quarter) without touching the previous one; (c) repeat on the last quarter (3, third quarter) by touching the previous one.
Figure A2
Figure A2
Comparison of subcultures of S. aureus on InTray MH Chocolate agar before (left) and after (right) optimisation of InTray subculturing protocol.
Figure 1
Figure 1
Mixed culture of Salmonella spp. (pink) and Pseudomonas aeruginosa (white) after subculture on InTray Colorex Screen (A) Visualization of mixed flora based on morphology; (B) Detection based on colony colour; (C) Mixed infection not detected.
See this image and copyright information in PMC

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