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. 2023 Jan 20;28(3):1036.
doi: 10.3390/molecules28031036.

Cupressus arizonica Greene: Phytochemical Profile and Cosmeceutical and Dermatological Properties of Its Leaf Extracts

Nora Tawfeek  1 , Eman Fikry  1 , Ismail Mahdi  2 , Melvin Adhiambo Ochieng  2 , Widad Ben Bakrim  2   3 , Noamane Taarji  2 , Mona F Mahmoud  4 , Mansour Sobeh  2
Affiliations

Cupressus arizonica Greene: Phytochemical Profile and Cosmeceutical and Dermatological Properties of Its Leaf Extracts

Nora Tawfeek et al. Molecules. .

Abstract

For many decades, natural resources have traditionally been employed in skin care. Here, we explored the phytochemical profile of the aqueous and ethanolic leaf extracts of Cupressus arizonica Greene and assessed their antioxidant, antiaging and antibacterial activities in vitro. Liquid chromatography-mass spectrometry (LC-MS/MS) analysis led to the tentative identification of 67 compounds consisting mainly of phenolic and fatty acids, diterpene acids, proanthocyanidins and flavonoid and biflavonoid glycosides. The aqueous extract demonstrated substantial in vitro antioxidant potential at FRAP and DPPH assays and inhibited the four target enzymes (collagenase, elastase, tyrosinase, and hyaluronidase) engaged in skin remodeling and aging with IC50 values close to those of the standard drugs. Moreover, the aqueous extract at 25 mg/mL suppressed biofilm formation by Pseudomonas aeruginosa, a bacterial pathogen causing common skin manifestations, and decreased its swarming and swimming motilities. In conclusion, C. arizonica leaves can be considered a promising candidate for potential application in skin aging.

Keywords: Cupressus arizonica Greene; biofilm inhibition; collagenase; cosmeceutical; dermatological; elastase; hyaluronidase; tyrosinase.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Effect of the different concentrations of aqueous and ethanolic extracts of C. arizonica leaves on bacterial growth rate of P. aeruginosa. Different letters in superscript indicate a statistical difference at p < 0.05.
Figure 2
Figure 2
Biofilm production by P. aeruginosa grown in the absence (0 mg/mL) and presence of the extracts at 12.5 mg/mL (1/8 MIC) and 25 mg/mL (1/4 MIC). Different letters in superscript indicate a statistical difference at p < 0.05.
Figure 3
Figure 3
Effect of sub-MICs of C. arizonica leaf extracts on the swarming (a) and swimming (b) motilities of P. aeruginosa on agar plates. Different letters in superscript indicate a statistical difference at p < 0.05.
Figure 4
Figure 4
HPLC-MS profile of aqueous extract (top) and ethanolic extract (bottom) from C. arizonica leaves.
See this image and copyright information in PMC

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