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. 2023 Jan 29;12(3):602.
doi: 10.3390/plants12030602.

Chromosomal Location of xa19, a Broad-Spectrum Rice Bacterial Blight Resistant Gene from XM5, a Mutant Line from IR24

Satoru Taura  1   2 , Katsuyuki Ichitani  2   3
Affiliations

Chromosomal Location of xa19, a Broad-Spectrum Rice Bacterial Blight Resistant Gene from XM5, a Mutant Line from IR24

Satoru Taura et al. Plants (Basel). .

Abstract

Bacterial blight is an important rice disease caused by bacteria named Xanthomonas oryzae pv. oryzae (Xoo). XM5 is an Xoo resistant mutant line with the genetic background of IR24, an Indica Xoo susceptible cultivar, induced by a chemical mutagen N-methyl-N-nitrosourea (MNU). XM5 carries a recessive Xoo resistant gene, xa19. Trisomic analysis was conducted using the cross between XM5 and the trisomic series under the genetic background of IR24, showing that xa19 was located on chromosome 7. The approximate chromosomal location was found using 37 surely resistant plants in the F2 population from XM5 × Kinmaze, which was susceptible to most Japanese Xoo races. The IAS44 line carries a Japonica cultivar Asominori chromosomal segment covering the xa19 locus under the IR24 genetic background. Linkage analysis using the F2 population from the cross between XM5 and IAS44 revealed that xa19 was located within the 0.8 cM region between RM8262 and RM6728. xa19 is not allelic to the known Xoo resistant genes. However, its location suggests that it might be allelic to a lesion-mimic mutant gene spl5, some alleles of which are resistant to several Xoo races. Together with xa20 and xa42, three Xoo resistant genes were induced from IR24 by MNU. The significance of chemical mutagen as a source of Xoo resistance was discussed.

Keywords: Oryza sativa; Xanthomonas oryzae pv. oryzae; mutation; resistance by a recessive gene; trisomic analysis.

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Conflict of interest statement

The authors declare no conflicts of interest. The funders had no role in the design of the study; in the collection, analyses, or interpretation of data; in the writing of the manuscript; or in the decision to publish the results.

Figures

Figure 1
Figure 1
Frequency distribution of lesion length at 18 days after inoculation with Xoo Japanese race II (T7147) of the F2 population from the cross between XM5 and IAS44. The classified genotypes were assessed for RM6728 as noted: black, homozygous for XM5; gray, heterozygous; white, homozygous for IAS44. XM5 (10 plants), IAS44 (5 plants) and IR24 (10 plants) were planted as reference, and their lesion length ranges are shown as stippled boxes on the top.
Figure 2
Figure 2
Linkage map of xa19 and surrounding DNA markers. (a,b) RFLP framework maps of chromosome 7 modified from Harushima et al. [47]. (c) the linkage map of xa19 gene constructed from F2 population from the cross between XM5 and IAS44 (n = 210). DNA markers located near each other on Os-Nipponbare-Reference-IRGSP-1.0 are connected by dotted lines.
See this image and copyright information in PMC

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