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. 2023 Jan 18;15(3):504.
doi: 10.3390/polym15030504.

Accelerated Simple Preparation of Curcumin-Loaded Silk Fibroin/Hyaluronic Acid Hydrogels for Biomedical Applications

Mohamed Chaala  1 , Fatima Zohra Sebba  1 , Marta G Fuster  2 , Imane Moulefera  2 , Mercedes G Montalbán  2 , Guzmán Carissimi  2 , Gloria Víllora  2
Affiliations

Accelerated Simple Preparation of Curcumin-Loaded Silk Fibroin/Hyaluronic Acid Hydrogels for Biomedical Applications

Mohamed Chaala et al. Polymers (Basel). .

Abstract

The development of new biomaterials from natural fibres in the field of biomedicine have attracted great interest in recent years. One of the most studied fibres has been silk fibroin produced by the Bombyx mori worm, due to its excellent mechanical properties and its biodegradability and bioavailability. Among the different biomaterials that can be prepared from silk fibroin, hydrogels have attracted considerable attention due to their potential use in different fields, such as scaffolding, cell therapy and biomedical application. Hydrogels are essentially a three-dimensional network of flexible polymer chains that absorb considerable amounts of water and can be loaded with drugs and/or cells inside to be used in a wide variety of applications. Here we present a simple sonication process for the preparation of curcumin-hyaluronic acid-silk fibroin hydrogels. Different grades of hydrogels were prepared by controlling the relative amounts of their components. The hydrogels were physically and morphologically characterised by Fourier transform infrared spectroscopy (FTIR), X-ray diffraction (XRD), thermogravimetric analysis (TGA) and field emission scanning electron microscopy (FESEM) and their biological activity was tested in terms of cell viability in a fibroblast cell line.

Keywords: cellular proliferation; curcumin; hyaluronic acid; hydrogel; silk fibroin.

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Conflict of interest statement

The authors declare no conflict of interest.

Figures

Figure 1
Figure 1
Schematic of the experimental set-up release apparatus.
Figure 2
Figure 2
Macroscopic appearance of (a) sample SF, (b) sample A2, (c) sample B3 and (d) sample C4.
Figure 3
Figure 3
ATR−FTIR spectra of SF fibre.
Figure 4
Figure 4
Amide I and II bands of silk fibroin.
Figure 5
Figure 5
ATR−FTIR absorbance spectra of (A) SF/HA hydrogels (A1, A2, A3, A5), (B) SF/curcumin hydrogels (B1, B2, B3, B5); (C) SF/HA/curcumin hydrogels with SF/HA = 80/20 and SF/curcumin 0, 2/1, 4/1 and 8/1 (A2, C1, C4, C7) and (D) SF/HA/curcumin hydrogels with SF/HA = 40/60 and SF/curcumin 0, 2/1, and 8/1 (A5, C3, C9).
Figure 6
Figure 6
Observationof the morphology by FESEM of SF (A1,B1), SF/HA (A2,A5) and SF/curcumin hydrogels (B1,B3,B5); SF/HA/curcumin hydrogels with SF/HA = 80/20 (C1,C4,C7) and SF/curcumin = 40/60 (C3,C6,C9).
Figure 7
Figure 7
Thermogravimetric analysis (TGA) and differential thermal analysis (DTA): (A) SF/HA; (B) curcumin-loaded SF; and (C) curcumin-loaded SF/HA hydrogels.
Figure 8
Figure 8
X-ray patterns of (A) SF/HA hydrogels; (B) curcumin-loaded SF; and (C) curcumin-loaded SF/HA hydrogels.
Figure 9
Figure 9
In vitro drug release profiles of curcumin powder and curcumin-loaded hydrogel made of SF and SF/HA with different mass ratio.
Figure 10
Figure 10
Cell density of (A) SF/HA; (B) curcumin-loaded SF; (C) curcumin-loaded SF/HA. Data are expressed as percentage of cell viability ± SD vs. concentration. * Indicates p < 0.05, ** indicates p < 0.01, and **** indicates p < 0.0001, compared to control.
Figure 11
Figure 11
Comparative results of cell density between (A) SF/HA (A1, A2 and A5), curcumin-loaded SF (B1, B3 and B5), and curcumin-loaded SF/HA hydrogels (C1, C3, C4, C6 and C7; (B) SF/HA (A5) and curcumin-loaded SF/HA hydrogels (C3 C6, and C9)); (C) SF/HA (A2) and curcumin-loaded SF/HA hydrogels (C1 C4, and C7). Data are expressed as percentage of cell viability ± SD vs. concentration. * Indicates p < 0.05, ** indicates p < 0.01, *** indicates p < 0.001 and **** indicates p < 0.0001, compared to control.
Figure 12
Figure 12
Morphological images of cells in the samples of Control, SF/HA (A1,A2) at three days and curcumin-loaded SF/HA hydrogels (C4,C7) at seven days.
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