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Review
. 2023:2637:27-39.
doi: 10.1007/978-1-0716-3016-7_2.

Updated Overview of TALEN Construction Systems

Tetsushi Sakuma  1 , Takashi Yamamoto  2
Affiliations
Review

Updated Overview of TALEN Construction Systems

Tetsushi Sakuma et al. Methods Mol Biol. 2023.

Abstract

Transcription activator-like effector (TALE) nuclease (TALEN) is the second-generation genome editing tool consisting of TALE protein containing customizable DNA-binding repeats and nuclease domain of FokI enzyme. Each DNA-binding repeat recognizes one base of double-strand DNA, and functional TALEN can be created by a simple modular assembly of these repeats. To easily and efficiently assemble the highly repetitive DNA-binding repeat arrays, various construction systems such as Golden Gate assembly, serial ligation, and ligation-independent cloning have been reported. In this chapter, we summarize the updated situation of these systems and publicly available reagents and protocols, enabling optimal selection of best suited systems for every researcher who wants to utilize TALENs in various research fields.

Keywords: Golden Gate assembly; Ligation-independent cloning; Pre-assembled library; Serial ligation; TALEN.

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References

    1. Sakuma T, Yamamoto T (2015) CRISPR/Cas9: the leading edge of genome editing technology. In: Yamamoto T (ed) Targeted genome editing using site-specific nucleases: ZFNs, TALENs, and the CRISPR/Cas9 system. Springer Japan, Tokyo, pp 25–41
    1. Sakuma T, Woltjen K (2014) Nuclease-mediated genome editing: at the front-line of functional genomics technology. Develop Growth Differ 56:2–13
    1. Boch J, Scholze H, Schornack S, Landgraf A, Hahn S, Kay S, Lahaye T, Nickstadt A, Bonas U (2009) Breaking the code of DNA binding specificity of TAL-type III effectors. Science 326:1509–1512
    1. Moscou MJ, Bogdanove AJ (2009) A simple cipher governs DNA recognition by TAL effectors. Science 326:1501
    1. Sakuma T, Ochiai H, Kaneko T, Mashimo T, Tokumasu D, Sakane Y, Suzuki K, Miyamoto T, Sakamoto N, Matsuura S, Yamamoto T (2013) Repeating pattern of non-RVD variations in DNA-binding modules enhances TALEN activity. Sci Rep 3:3379

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