Genome Editing of Rat
- PMID: 36773150
- DOI: 10.1007/978-1-0716-3016-7_17
Genome Editing of Rat
Abstract
Many genetically engineered rat strains have been produced by the development of genome editing technology, although it used to be technical difficulty and low production efficiency. Knockout and knock-in strains can be simple and quick produced using zinc finger nuclease (ZFN), transcription activator-like effector nuclease (TALEN), or clustered regularly interspaced short palindromic repeats (CRISPR)-Cas9. Presently, genome edited strains have been produced by microinjection and a new electroporation method named technique for animal knockout system by electroporation (TAKE). This chapter presents the latest protocols for producing genome edited rats.
Keywords: CRISPR-Cas9; Electroporation; Embryo transfer; Embryos; In vitro fertilization; Microinjection; Rat.
© 2023. The Author(s), under exclusive license to Springer Science+Business Media, LLC, part of Springer Nature.
Similar articles
-
Genome Editing of Rat.Methods Mol Biol. 2017;1630:101-108. doi: 10.1007/978-1-4939-7128-2_9. Methods Mol Biol. 2017. PMID: 28643253
-
Genome Editing in Mouse and Rat by Electroporation.Methods Mol Biol. 2017;1630:81-89. doi: 10.1007/978-1-4939-7128-2_7. Methods Mol Biol. 2017. PMID: 28643251
-
Genome Editing in Mouse and Rat by Electroporation.Methods Mol Biol. 2023;2637:125-134. doi: 10.1007/978-1-0716-3016-7_10. Methods Mol Biol. 2023. PMID: 36773143
-
Recent Advances in the Production of Genome-Edited Rats.Int J Mol Sci. 2022 Feb 25;23(5):2548. doi: 10.3390/ijms23052548. Int J Mol Sci. 2022. PMID: 35269691 Free PMC article. Review.
-
Genome editing: the road of CRISPR/Cas9 from bench to clinic.Exp Mol Med. 2016 Oct 14;48(10):e265. doi: 10.1038/emm.2016.111. Exp Mol Med. 2016. PMID: 27741224 Free PMC article. Review.
Cited by
-
Advances in CRISPR/Cas-Based Strategies on Zoonosis.Transbound Emerg Dis. 2023 Aug 3;2023:9098445. doi: 10.1155/2023/9098445. eCollection 2023. Transbound Emerg Dis. 2023. PMID: 40303816 Free PMC article. Review.
-
Methods for Modeling Early Life Stress in Rodents.Methods Mol Biol. 2025;2868:205-219. doi: 10.1007/978-1-0716-4200-9_11. Methods Mol Biol. 2025. PMID: 39546232 Review.
References
-
- Geurts AM, Cost GJ, Freyvert Y et al (2009) Knockout rats produced via embryo pronuclear microinjection of designed zinc finger nucleases. Science 325:433. https://doi.org/10.1126/science.1172447 - DOI
-
- Tesson L, Usal C, Ménoret S et al (2011) Knockout rats generated by embryo microinjection of TALENs. Nat Biotechnol 29:695–696. https://doi.org/10.1038/nbt.1940 - DOI
-
- Li D, Qiu Z, Shao Y et al (2013) Heritable gene targeting in the mouse and rat using a CRISPR-Cas system. Nat Biotechnol 31:681–683. https://doi.org/10.1038/nbt.2661 - DOI
-
- Li W, Teng F, Li T et al (2013) Simultaneous generation and germline transmission of multiple gene mutations in rat using CRISPR-Cas systems. Nat Biotechnol 31:684–686. https://doi.org/10.1038/nbt.2652 - DOI
-
- Tong C, Li P, Wu NL et al (2010) Production of p53 gene knockout rats by homologous recombination in embryonic stem cells. Nature 467:211–213. https://doi.org/10.1038/nature09368 - DOI
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
