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. 2023 Feb 11;168(3):86.
doi: 10.1007/s00705-023-05708-7.

Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus

Jing Zhou  1   2   3 , Xiaojun Hu  1 , Paulo Vieira  4 , Benjamin Atha 3rd  1   5 , Clint McFarland  6 , Joseph A Foster  1 , Oscar P Hurtado-Gonzales  7
Affiliations

Molecular characterization of horse nettle virus A, a new member of subgroup B of the genus Nepovirus

Jing Zhou et al. Arch Virol. .

Abstract

A new positive-strand RNA virus was discovered in a horse nettle plant, using high-throughput sequencing (HTS), and its complete genome, consisting of RNA1 and RNA2, which are 7522 and 4710 nucleotides in length, respectively, was characterized. Each genome segment contains a single open reading frame flanked by 5' and 3' untranslated regions (UTRs), followed by a poly(A) tail at the 3' end. The encoded proteins have the highest amino acid sequence identity (55% and 45%) to the polyprotein encoded by RNA1 of tomato black ring virus (TBRV) and RNA2 of potato virus B (PVB), respectively. Its genome organization and phylogenetic relationship to other nepoviruses suggested that this virus is a novel member of subgroup B, and recombination analysis revealed its evolutionary history within the subgroup. These results suggest the new virus, provisionally named "horse nettle virus A", represents a new species within the genus Nepovirus.

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