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. 2023 Jan 26:13:1076500.
doi: 10.3389/fonc.2023.1076500. eCollection 2023.

Intraoperative oxygen tension and redox homeostasis in Pseudomyxoma peritonei: A short case series

Francisca Valenzuela-Molina  1   2 , Florina I Bura  2 , Mari C Vázquez-Borrego  2 , Melissa Granados-Rodríguez  2 , Blanca Rufián-Andujar  1   2 , Sebastián Rufián-Peña  1   2 , Ángela Casado-Adam  1   2 , Juan Manuel Sánchez-Hidalgo  1   2 , Lidia Rodríguez-Ortiz  1   2 , Rosa Ortega-Salas  2   3 , Ana Martínez-López  2   3 , Carmen Michán  2 , José Alhama  2 , Álvaro Arjona-Sánchez  1   2 , Antonio Romero-Ruiz  2
Affiliations

Intraoperative oxygen tension and redox homeostasis in Pseudomyxoma peritonei: A short case series

Francisca Valenzuela-Molina et al. Front Oncol. .

Abstract

Introduction: Pseudomyxoma peritonei (PMP) is a rare malignant disease characterized by a massive multifocal accumulation of mucin within the peritoneal cavity. The current treatment option is based on complete cytoreductive surgery combined with hyperthermic intraperitoneal chemotherapy. However, the recurrence is frequent with subsequent progression and death. To date, most of the studies published in PMP are related to histological and genomic analyses. Thus, the need for further studies unveiling the underlying PMP molecular mechanisms is urgent. In this regard, hypoxia and oxidative stress have been extensively related to tumoral pathologies, although their contribution to PMP has not been elucidated.

Methods: In this manuscript, we have evaluated, for the first time, the intratumoral real-time oxygen microtension (pO2mt) in the tumor (soft and hard mucin) and surrounding healthy tissue from five PMP patients during surgery. In addition, we measured hypoxia (Hypoxia Inducible Factor-1a; HIF-1α) and oxidative stress (catalase; CAT) markers in soft and hard mucin from the same five PMP patient samples and in five control samples.

Results: The results showed low intratumoral oxygen levels, which were associated with increased HIF-1α protein levels, suggesting the presence of a hypoxic environment in these tumors. We also found a significant reduction in CAT activity levels in soft and hard mucin compared with healthy tissue samples.

Discussion: In conclusion, our study provides the first evidence of low intratumoral oxygen levels in PMP patients associated with hypoxia and oxidative stress markers. However, further investigation is required to understand the potential role of oxidative stress in PMP in order to find new therapeutic strategies.

Keywords: HIF-1α; Pseudomyxoma peritonei; ROS; catalase; hypoxia; mucin; oxygen.

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Conflict of interest statement

The authors declare that the research was conducted in the absence of any commercial or financial relationships that could be construed as a potential conflict of interest.

Figures

Figure 1
Figure 1
Measurement of intraoperative real-time oxygen microtension (pO2mt) in blood and tissues during surgery. (A) Individual measurements of pO2mt (mmHg) in blood (B), surrounding healthy tissue (HT), soft mucin (SM) and hard mucin (HM) in each PMP patient (n=5). Measurements are represented as the mean ± S.E.M of all values measured per zone in each patient. (B) pO2mt (mmHg) levels per zone taking into account all patients included in the study (n=5). One-way ANOVA analysis was carried out with multiple comparisons. *p < 0.05, ***p < 0.001.
Figure 2
Figure 2
Cellular hypoxia and oxidative stress markers in PMP samples. (A) HIF-1α relative protein expression levels in soft (SM; n=5) and hard mucin (HM; n=5) compared with healthy tissues (HT; n=5; non-tumoral appendix and colon tissues) evaluated by Western Blot. The arbitrary densitometric unit (ADU) for the protein was normalized by the Total Protein Normalization (TPN) value. (B) Catalase activity (U/mg) in soft and hard mucin (n=5) compared to healthy control tissues (n=5; non-tumoral appendix and colon tissues) evaluated by enzymatic assay. Unpaired t test or Mann-Whitney test was used based on Kolmogorov-Smirnov normality test. *p < 0.05, **p < 0.01.
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