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Review
. 2023 Feb 3:1-17.
doi: 10.1007/s12257-022-0268-5. Online ahead of print.

Cell-free Biosynthesis of Peptidomimetics

Kanghun Lee #  1 , Jessica A Willi #  2 , Namjin Cho  3 , Inseon Kim  1 , Michael C Jewett  2   4 , Joongoo Lee  1   3
Affiliations
Review

Cell-free Biosynthesis of Peptidomimetics

Kanghun Lee et al. Biotechnol Bioprocess Eng. .

Abstract

A wide variety of peptidomimetics (peptide analogs) possessing innovative biological functions have been brought forth as therapeutic candidates through cell-free protein synthesis (CFPS) systems. A key feature of these peptidomimetic drugs is the use of non-canonical amino acid building blocks with diverse biochemical properties that expand functional diversity. Here, we summarize recent technologies leveraging CFPS platforms to expand the reach of peptidomimetics drugs. We also offer perspectives on engineering the translational machinery that may open new opportunities for expanding genetically encoded chemistry to transform drug discovery practice beyond traditional boundaries.

Keywords: cell-free protein synthesis systems; non-canonical amino acids; peptidomimetics; translational machinery.

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Conflict of interest statement

M.C.J. is a cofounder of SwiftScale Biologics, Stemloop, Inc., Design Pharmaceuticals, and Pearl Bio. The interests of M.C.J. are reviewed and managed by Northwestern University in accordance with their competing interest policies. All other authors declare no competing interests. Neither ethical approval nor informed consent was required for this study.

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