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. 2023 Apr;50(4):3493-3502.
doi: 10.1007/s11033-023-08305-8. Epub 2023 Feb 13.

Comparative transcriptome analysis of Saposhnikovia divaricata to reveal drought and rehydration adaption strategies

Youla Su  1   2   3 , Miaomiao Jiao  1   2   3 , Huan Guan  1   2   3 , Yuhuan Zhao  1   2   3 , Cuomu Deji  1   2 , Guilin Chen  4   5   6
Affiliations

Comparative transcriptome analysis of Saposhnikovia divaricata to reveal drought and rehydration adaption strategies

Youla Su et al. Mol Biol Rep. 2023 Apr.

Abstract

Background: Water scarcity has become one of the most prevalent environmental factors adversely affecting plant growth and development. Different species have developed multiple ways of drought resistance. Saposhnikovia divaricata is a commonly used traditional herb in East Asia. However, limited information is available on the drought response of this herb and further clarification of underlying molecular mechanism remains a challenge.

Methods and results: In this study, a comparative transcriptome analysis was firstly conducted to identify the major pathways and candidate genes involved in the drought adaptive response of S. divaricata. The seedlings of S. divaricata were subjected to progressive drought by withholding water for 16 days followed by 8 days of rehydration. Transcriptome analysis identified a total of 89,784 annotated unigenes. The number of differentially expressed genes (DEGs) gradually increased with the deepening of drought and decreased after rehydration. Gene Ontology enrichment analysis and Kyoto Encyclopedia of Genes and Genomes pathway analysis suggested genes related to oxidoreductase activity, carbohydrate metabolism, plant hormone signaling pathway and secondary metabolism were important in drought response of S. divaricata. Specific genes involved in reactive oxygen species scavenging system (POD, Cu/Zn-SOD, APX), abscisic acid and jasmonic acid signaling pathway (PYL4, PP2Cs, JAR1, JAZ) and phenylpropanoid biosynthesis (4CL, CCR, CAD) underwent dynamic alterations under drought and rehydration. Finally, the expression pattern of 12 selected DEGs from the transcriptomic profiling was validated by real-time quantitative PCR.

Conclusion: Our study laid a foundation for understanding the stress response of S. divaricata and other Apiaceae family plant at molecular level.

Keywords: Drought; Hormonal pathway; Phenylpropanoid; Saposhnikovia divaricata; Transcriptome.

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