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. 2023:2638:165-171.
doi: 10.1007/978-1-0716-3024-2_11.

Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Yang Zhou  1 , Huitang Pan  2
Affiliations

Specific-Locus Amplified Fragment Sequencing (SLAF-Seq)

Yang Zhou et al. Methods Mol Biol. 2023.

Abstract

Specific length amplified fragment sequencing (SLAF-seq) technology is a simplified genome sequencing technology based on next-generation sequencing. SLAF-seq technology has several distinguishing characteristics: 1. Deep sequencing to ensure accuracy of genotyping; 2. Effectively reduce sequencing costs; 3. Pre-designed simplified representation scheme to optimize marker efficiency; 4. Doubled barcode system for large populations. The advantages and technical process of SLAF-seq are described briefly with summarized results for the application of SLAF-seq in development of molecular markers, construction of high-density genetic map and gene mapping in ornamental plants. Finally, the difficulties and prospects of this method are discussed in application.

Keywords: Genotyping; Molecular markers; SLAF; SNP.

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