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. 2023:2632:259-267.
doi: 10.1007/978-1-0716-2996-3_18.

Long-Read Single-Cell Sequencing Using scCOLOR-seq

Martin Philpott  1 , Udo Oppermann  1   2 , Adam P Cribbs  3   4
Affiliations

Long-Read Single-Cell Sequencing Using scCOLOR-seq

Martin Philpott et al. Methods Mol Biol. 2023.

Abstract

Single-cell sequencing allows for the measurement of sequence information from individual cells with next-generation sequencing (NGS). However, its application to third-generation sequencing platforms such as Oxford Nanopore has been challenging because of its lower basecalling accuracy. Here we describe the method to perform highly accurate single-cell COrrected Long-Read sequencing (scCOLOR-seq) by droplet-based encapsulation of cells and sequencing using the Oxford Nanopore Sequencing system.

Keywords: Long-read; Oxford Nanopore Sequencing; Single-cell.

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References

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