Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023:2628:41-49.
doi: 10.1007/978-1-0716-2978-9_4.

Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Research Analysis

Rosemary L Sparrow  1   2 , Richard J Simpson  3 , David W Greening  4   5   6   7
Affiliations

Preparation of Cryoprecipitate and Cryo-depleted Plasma for Proteomic Research Analysis

Rosemary L Sparrow et al. Methods Mol Biol. 2023.

Abstract

When frozen plasma is slowly thawed in cold conditions (1-6 °C), high-molecular-weight plasma proteins precipitate forming a concentrate known as cryoprecipitate. The concentrate is enriched with several important coagulation proteins, including fibrinogen, antihemophilic factor (factor VIII), von Willebrand factor, fibrin stabilizing factor (factor XIII), fibronectin, and small amounts of other plasma proteins. In current medical practice, clinical-grade preparations of cryoprecipitate are used mostly to correct fibrinogen deficiency caused by acute blood loss or due to functional abnormalities of the fibrinogen protein. In the past, cryoprecipitate was used to treat von Willebrand disease and hemophilia A (factor VIII deficiency), but the availability of more highly purified coagulation factor concentrates or recombinant protein preparations has superseded the use of cryoprecipitate for these coagulopathies. Cryo-depleted plasma (also called cryosupernatant) is the plasma supernatant remaining following removal of the cryoprecipitate from frozen-thawed plasma and contains all the remaining soluble plasma proteins. This protocol describes the research-scale preparation of cryoprecipitate and cryo-depleted plasma suitable for proteomic studies and is based on the procedures used to prepare clinical-grade cryoprecipitate.

Keywords: Antihemophilic factor; Cryo-depleted; Cryoprecipitate; Cryosupernatant; Factor VIII; Fibrinogen; Plasma.

PubMed Disclaimer

References

    1. Pool JG, Gershgold EJ, Pappenhagen AR (1964) High-potency antihaemophilic factor concentrate prepared from cryoglobulin precipitate. Nature 203:312 - DOI
    1. Pool JG (1965) Preparation and testing of antihemophilic globulin (Factor 8) sources for transfusion therapy in hemophilia. Description of a new sterile concentrate process for blood banks. Scand J Clin Lab Invest 17(Suppl 84):70–87
    1. Callum JL, Karkouti K, Lin Y (2009) Cryoprecipitate: the current state of knowledge. Transfus Med Rev 23(3):177–188 - DOI
    1. Nair PM, Rendo MJ, Reddoch-Cardenas KM, Burris JK, Meledeo MA, Cap AP (2020) Recent advances in use of fresh frozen plasma, cryoprecipitate, immunoglobulins, and clotting factors for transfusion support in patients with hematologic disease. Semin Hematol 57(2):73–82. https://doi.org/10.1053/j.seminhematol.2020.07.006 - DOI
    1. Mason EC (1978) Thaw-siphon technique for production of cryoprecipitate concentrate of factor VIII. Lancet 2(8079):15–17 - DOI

LinkOut - more resources