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Safety evaluation of the food enzyme d-tagatose 3-epimerase from the genetically modified Escherichia coli strain PS-Sav-001

EFSA Panel on Food Contact Materials, Enzymes and Processing Aids (CEP) et al. EFSA J. .

Abstract

The food enzyme d-tagatose 3-epimerase (EC 5.1.3.31) is produced with the genetically modified Escherichia coli strain PS-Sav-001 by SAVANNA Ingredients GmbH. The genetic modifications do not give rise to safety concerns. The food enzyme is considered free from viable cells of the production organism and its DNA. The food enzyme is used while retained inside a membrane reactor to convert d-fructose into the speciality carbohydrate d-allulose (syn. d-psicose). Since residual amounts of total organic solids (TOS) are removed by the purification steps applied during the production of d-allulose, dietary exposure was not calculated and toxicological studies were not considered necessary. A search for the similarity of the amino acid sequence of the food enzyme to known allergens was made and no match was found. The Panel considered that under the intended conditions of use, the risk of allergic reactions by dietary exposure cannot be excluded, but the likelihood is low. Based on the data provided, the Panel concluded that this food enzyme does not give rise to safety concerns under the intended conditions of use.

Keywords: EC 5.1.3.31; Escherichia coli; d‐tagatose 3‐epimerase; food enzyme; genetically modified microorganism; l‐ribulose 3‐epimerase.

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References

    1. Chart H, Smith HR, La Ragione RM and Woodward MJ, 2000. An investigation into the pathogenic properties of Escherichia coli strains BLR, BL21, DH5alpha and EQ1. Journal of Applied Microbiology, 89, 1048–1058. 10.1046/j.1365-2672.2000.01211.x - DOI - PubMed
    1. Daegelen P, Studier FW, Lenski RE, Cure S and Kim JF, 2009. Tracing ancestors and relatives of Escherichia coli B, and the derivation of B Strains REL606 and BL21(DE3). Journal of Molecular Biology, 394, 634–643. 10.1016/j.jmb.2009.09.022 - DOI - PubMed
    1. EFSA (European Food Safety Authority) , 2009a. Guidance of EFSA prepared by the Scientific Panel of Food Contact Material, Enzymes, Flavourings and Processing Aids on the Submission of a Dossier on Food Enzymes. EFSA Journal 2009;7(8):1305, 26 pp. 10.2903/j.efsa.2009.1305 - DOI
    1. EFSA (European Food Safety Authority) , 2009b. Guidance of the Scientific Committee on transparency in the scientific aspects of risk assessments carried out by EFSA. Part 2: general principles. EFSA Journal 2009;7(5):1051, 22 pp. 10.2903/j.efsa.2009.1051 - DOI
    1. EFSA CEF Panel (EFSA Panel on Food Contact Materials, Enzymes, Flavourings and Processing Aids) , 2016. Panel statement on the exposure assessment of food enzymes. EFSA Journal 2016;14(11):4581, 9 pp. 10.2903/j.efsa.2016.4581 and Annex B – Process‐specific technical data used in exposure assessment of food enzymes (Available online: https://efsa.onlinelibrary.wiley.com/action/downloadSupplement?doi=10.29...) - DOI - PubMed

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