A noninvasive test for estimating myosin heavy chain of the vastus lateralis in females with mechanomyography

Abstract

This study examined relationships between percent myosin heavy chain (%MHC) expression and mechanomyographic amplitude (MMG_RMS). Fifteen females (age ± SD=21.3 ± 5.3 yrs) completed isometric trapezoidal contractions at 30% and 70% maximal voluntary contraction (MVC). MMG was recorded from the vastus lateralis (VL). Participants gave a muscle biopsy of the VL post-testing. MMG_RMS-torque relationships during the linearly varying segments were log-transformed and linear regressions were applied to calculate b terms (slopes). For the steady torque segment, MMG_RMS was averaged. Correlations were performed for type I%MHC with the MMG variables. Multiple regression was utilized to examine prediction equations for type I%MHC. Type I%MHC was significantly correlated with the b terms during the increasing segment of the 70% MVC (p = 0.003; r = -0.718), and MMG_RMS during steady torque at 30% (p = 0.008; r = -0.652) and 70% MVC (p = 0.040; r = -0.535). Type I%MHC reduced the linearity of the MMG_RMS-torque relationship during the high-intensity linearly increasing segment, and MMG_RMS at a low- and high-intensity steady torque. A combination of MMG variables estimated type I%MHC expression with 81.2% accuracy. MMG recorded during a low- and high-intensity isometric trapezoidal contraction may offer a simple, noninvasive test for estimating type I%MHC expression of the VL in sedentary females.

Keywords: Females; Fiber type; Isometric trapezoidal contraction; Log-transform; Mechanomyography; Motor unit; Myosin heavy chain; Vastus lateralis.