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. 2023:2612:59-71.
doi: 10.1007/978-1-0716-2903-1_5.

Effective Blocking and Stabilizing Methods Using Synthetic Polymer on ELISA

Tomozumi Noda  1 , Masaru Matsuda  1 , Hirotaka Suzuki  1 , Yusuke Okawa  1 , Motohiro Mitani  1
Affiliations

Effective Blocking and Stabilizing Methods Using Synthetic Polymer on ELISA

Tomozumi Noda et al. Methods Mol Biol. 2023.

Abstract

In ELISA, blocking reagents and stabilizers are important to improve the sensitivity and/or quantitative nature of the measurement system. Usually, biological substances such as bovine serum albumin and casein are used, but they still have problems such as lot-to-lot differences and biohazard. Here, we describe the methods using a chemically synthesized polymer, BIOLIPIDURE®, as a new blocking agent and stabilizer that can solve these problems.

Keywords: Antibody; Blocking; Chemically synthesized materials; ELISA; Stabilizer.

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