K562--a human erythroleukemic cell line
- PMID: 367973
- DOI: 10.1002/ijc.2910230202
K562--a human erythroleukemic cell line
Abstract
We have studied the surface membrane properties of the human leukemic cell line K562 which previously has been reported to represent an early stage of granulocyte maturation. The surface glycoprotein pattern of the K562 cells obtained after galactose oxidase-NaB[3H]4 labelling and slab gel electrophoresis shows striking similarities with that of normal erythrocytes but is completely different from the patterns of normal and malignant cells of various stages of the myeloblast to granulocyte differentiation. Moreover, the K562 cell expressed the major red cell sialoglycoprotein, glycophorin, on its surface as shown by immunofluorescence and by immunoprecipitation from labelled membrane preparations. As glycophorin is exclusively found on erythroid cells in human bone marrow we conclude that the K562 is a human erythroleukemic line.
Similar articles
-
Differentiation of human erythroid cells is associated with increased O-glycosylation of the major sialoglycoprotein, glycophorin A.Proc Natl Acad Sci U S A. 1984 Nov;81(21):6752-6. doi: 10.1073/pnas.81.21.6752. Proc Natl Acad Sci U S A. 1984. PMID: 6387712 Free PMC article.
-
Tumor-promoting phorbol diester-induced specific changes in cell surface glycoprotein profile of K562 human leukemic cells.Cancer Res. 1981 Nov;41(11 Pt 1):4621-8. Cancer Res. 1981. PMID: 7306980
-
Expression of the major red cell sialoglycoprotein, glycophorin A, in the human leukemic cell line K562.J Biol Chem. 1979 Aug 10;254(15):7442-8. J Biol Chem. 1979. PMID: 379005
-
K562--a human leukemia cell line with erythroid features.Semin Hematol. 1981 Jan;18(1):72-7. Semin Hematol. 1981. PMID: 7010612 Review. No abstract available.
-
Surface glycoproteins of malignant human leukocytes.Biochim Biophys Acta. 1982 Apr 29;651(2-3):65-83. doi: 10.1016/0304-419x(82)90007-5. Biochim Biophys Acta. 1982. PMID: 6284215 Review. No abstract available.
Cited by
-
Loss of natural killer activity as an indicator of relapse in acute leukaemia.Clin Exp Immunol. 1990 May;80(2):241-6. doi: 10.1111/j.1365-2249.1990.tb05241.x. Clin Exp Immunol. 1990. PMID: 2357852 Free PMC article.
-
Cell lines and peripheral blood leukocytes derived from individuals with chronic myelogenous leukemia display virtually identical proteins phosphorylated on tyrosine residues.Proc Natl Acad Sci U S A. 1987 Jul;84(13):4408-12. doi: 10.1073/pnas.84.13.4408. Proc Natl Acad Sci U S A. 1987. PMID: 2440021 Free PMC article.
-
Monoclonal antibody 3F8 recognises the neural cell adhesion molecule (NCAM) in addition to the ganglioside GD2.Br J Cancer. 1989 Dec;60(6):861-6. doi: 10.1038/bjc.1989.380. Br J Cancer. 1989. PMID: 2481486 Free PMC article.
-
Toll-like receptor 4 mediates the antitumor host response induced by a 55-kilodalton protein isolated from Aeginetia indica L., a parasitic plant.Clin Diagn Lab Immunol. 2004 May;11(3):483-95. doi: 10.1128/CDLI.11.3.483-495.2004. Clin Diagn Lab Immunol. 2004. PMID: 15138173 Free PMC article.
-
Mutation or not, what directly establishes a neoplastic state, namely cellular immortality and autonomy, still remains unknown and should be prioritized in our research.J Cancer. 2022 Jul 4;13(9):2810-2843. doi: 10.7150/jca.72628. eCollection 2022. J Cancer. 2022. PMID: 35912015 Free PMC article. Review.
MeSH terms
Substances
LinkOut - more resources
Full Text Sources
Other Literature Sources
Research Materials
