Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 May-Jun;39(3):e3335.
doi: 10.1002/btpr.3335. Epub 2023 Feb 28.

Modeling the effect of oxidative stress on Bordetella pertussis fermentations

Michael Vitelli  1 , Ibrahim Melih Tamer  2 , Mark Pritzker  1 , Hector Budman  1
Affiliations

Modeling the effect of oxidative stress on Bordetella pertussis fermentations

Michael Vitelli et al. Biotechnol Prog. 2023 May-Jun.

Abstract

A mathematical model is proposed for Bordetella pertussis with the main goal to better understand and describe the relation between cell growth, oxidative stress and NADPH levels under different oxidative conditions. The model is validated with flask experiments conducted under different conditions of oxidative stress induced by high initial glutamate concentrations, low initial inoculum and secondary culturing following exposure to starvation. The model exhibited good accuracy when calibrated and validated for the different experimental conditions. From comparisons of model predictions to data with different model mechanisms, it was concluded that intracellular reactive oxidative species only have an indirect effect on growth rate by reacting with NADPH and thereby reducing the amount of NADPH that is available for growth.

Keywords: flow cytometry; mechanistic model; reactive oxidative species; shake flask experiments; starvation.

PubMed Disclaimer

References

REFERENCES

    1. Bordet J, Gengou O. Le microbe de la coqueluche. Ann I'Institut Pasteur. 1906;20:731-774.
    1. Zavatti V. Monitoring of an Antigen Manufacturing Process Using Fluorescence (Master's thesis). University of Waterloo. 2014.
    1. Singh R, Mailloux RJ, Puiseux-Dao S, Appanna VD. Oxidative stress evokes a metabolic adaptation that favors increased NADPH synthesis and decreased NADH production in Pseudomonas fluorescens. J Bacteriol. 2007;189(18):6665-6675.
    1. Zavatti V. Application of Flow Cytometry and Fluorescence Spectroscopy to Monitor and Predict the Fermentation Activity in a Vaccine Manufacturing Process. 2019.
    1. Zheng W, Zhao Y, Zhang M, Wei Z, Miao K, Sun W. Oxidative stress response of Inonotus obliquus induced by hydrogen peroxide. Med Mycol. 2009;47(8):814-823.

Publication types

LinkOut - more resources