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. 2023 Apr 11;62(16):e202218413.
doi: 10.1002/anie.202218413. Epub 2023 Mar 8.

A Dual-Metal-Catalyzed Sequential Cascade Reaction in an Engineered Protein Cage

Paul Ebensperger  1 , Mariia Zmyslia  1 , Philipp Lohner  1 , Judith Braunreuther  1 , Benedikt Deuringer  2 , Anita Becherer  3 , Regine Süss  2 , Anna Fischer  3 , Claudia Jessen-Trefzer  1
Affiliations

A Dual-Metal-Catalyzed Sequential Cascade Reaction in an Engineered Protein Cage

Paul Ebensperger et al. Angew Chem Int Ed Engl. .

Abstract

Herein, we describe the creation of an artificial protein cage housing a dual-metal-tagged guest protein that catalyzes a linear, two-step sequential cascade reaction. The guest protein consists of a fusion protein of HaloTag and monomeric rhizavidin. Inside the protein capsid, we established a ruthenium-catalyzed allylcarbamate deprotection reaction followed by a gold-catalyzed ring-closing hydroamination reaction that led to indoles and phenanthridines with an overall yield of up to 66 % in aqueous solutions. Furthermore, we show that the encapsulation stabilizes the metal catalysts against deactivation by air, proteins and cell lysate.

Keywords: Encapsulins; Organometallic Catalysis; Prodrug Activation; Protein Capsids; Sequential Cascade Reactions.

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References

    1. R. A. J. F. Oerlemans, S. B. P. E. Timmermans, J. C. M. van Hest, ChemBioChem 2021, 22, 2051-2078.
    1. S. H. Petrosko, R. Johnson, H. White, C. A. Mirkin, J. Am. Chem. Soc. 2016, 138, 7443-7445.
    1. Q. Zhang, L. Catti, K. Tiefenbacher, Acc. Chem. Res. 2018, 51, 2107-2114.
    1. T. G. W. Edwardson, M. D. Levasseur, S. Tetter, A. Steinauer, M. Hori, D. Hilvert, Chem. Rev. 2022, 122, 9145-9197.
    1. H. Ren, S. Zhu, G. Zheng, Int. J. Mol. Sci. 2019, 20, 592.

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