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. 2023 Jun;238(6):2561-2577.
doi: 10.1111/nph.18734. Epub 2023 Feb 20.

DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus

Julien Vigneaud #  1 , Annegret Kohler #  2 , Mamadou Dia Sow  1 , Alain Delaunay  1 , Laure Fauchery  2 , Frederic Guinet  2 , Christian Daviaud  3 , Kerrie W Barry  4 , Keykhosrow Keymanesh  4 , Jenifer Johnson  4 , Vasanth Singan  4 , Igor Grigoriev  4   5 , Régis Fichot  1 , Daniel Conde  6 , Mariano Perales  6   7 , Jörg Tost  3 , Francis M Martin  2 , Isabel Allona  6   7 , Steven H Strauss  8 , Claire Veneault-Fourrey  2 , Stéphane Maury  1
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Free article

DNA hypomethylation of the host tree impairs interaction with mutualistic ectomycorrhizal fungus

Julien Vigneaud et al. New Phytol. 2023 Jun.
Free article

Abstract

Ectomycorrhizas are an intrinsic component of tree nutrition and responses to environmental variations. How epigenetic mechanisms might regulate these mutualistic interactions is unknown. By manipulating the level of expression of the chromatin remodeler DECREASE IN DNA METHYLATION 1 (DDM1) and two demethylases DEMETER-LIKE (DML) in Populus tremula × Populus alba lines, we examined how host DNA methylation modulates multiple parameters of the responses to root colonization with the mutualistic fungus Laccaria bicolor. We compared the ectomycorrhizas formed between transgenic and wild-type (WT) trees and analyzed their methylomes and transcriptomes. The poplar lines displaying lower mycorrhiza formation rate corresponded to hypomethylated overexpressing DML or RNAi-ddm1 lines. We found 86 genes and 288 transposable elements (TEs) differentially methylated between WT and hypomethylated lines (common to both OX-dml and RNAi-ddm1) and 120 genes/1441 TEs in the fungal genome suggesting a host-induced remodeling of the fungal methylome. Hypomethylated poplar lines displayed 205 differentially expressed genes (cis and trans effects) in common with 17 being differentially methylated (cis). Our findings suggest a central role of host and fungal DNA methylation in the ability to form ectomycorrhizas including not only poplar genes involved in root initiation, ethylene and jasmonate-mediated pathways, and immune response but also terpenoid metabolism.

Keywords: DEMETER; DNA METHYLATION 1; DNA demethylatione; Laccaria bicolor; mycorrhizas; pigenetic; poplar.

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References

    1. Abdulsalam O, Wagner K, Wirth S, Kunert M, David A, Kallenbach M, Boland W, Kothe E, Krause K. 2021. Phytohormones and volatile organic compounds, like geosmin, in the ectomycorrhiza of Tricholoma vaccinum and Norway spruce (Picea abies). Mycorrhiza 31: 173-188.
    1. Akalin A, Kormaksson M, Li S, Garrett-Bakelman FE, Figueroa ME, Melnick A, Mason CE. 2012. methylKit: a comprehensive R package for the analysis of genome-wide DNA methylation profiles. Genome Biology 13: R87.
    1. Alonso C, Ramos-Cruz D, Becker C. 2018. The role of plant epigenetics in biotic interactions. New Phytologist 221: 731-737.
    1. Balestrini R, Kottke I. 2016. Structure and development of ectomycorrhizal roots. In: Martin F, ed. Molecular mycorrhizal symbiosis. Hoboken, NJ, USA: John Wiley & Sons, 47-61.
    1. Basso V, Kohler A, Miyauchi S, Singan V, Guinet F, Šimura J, Novák O, Barry KW, Amirebrahimi M, Block J et al. 2020. An ectomycorrhizal fungus alters sensitivity to jasmonate, salicylate, gibberellin, and ethylene in host roots. Plant, Cell & Environment 43: 1047-1068.

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