A Chromosome-length Assembly of the Black Petaltail (Tanypteryx hageni) Dragonfly

Ethan R Tolman^{1

2}, Christopher D Beatty³, Jonas Bush⁴, Manpreet Kohli^{1

5}, Carlos M Moreno⁶, Jessica L Ware¹, K Scott Weber, Ruqayya Khan⁷, Chirag Maheshwari⁷, David Weisz⁷, Olga Dudchenko^{7

8}, Erez Lieberman Aiden^{7

9

8

10

11

12}, Paul B Frandsen^{4

12

13}

Affiliations

¹ American Museum of Natural History, Department of Invertebrate Zoology, New York, New York.
² The City University of New York Graduate Center, New York, New York.
³ Program for Conservation Genomics, Department of Biology, Stanford University.
⁴ Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah.
⁵ Department of Natural Sciences, Baruch College, New York, New York.
⁶ Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah.
⁷ The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.
⁸ The University of Western Australia, UWA School of Agriculture and Environment, Crawley, Washington, Australia.
⁹ Center for Theoretical Biological Physics and Department of Computer Science, Rice University, Houston, Texas.
¹⁰ Broad Institute of MIT and Harvard, Cambridge, Massachusetts.
¹¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech, Pudong, China.
¹² LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt, Germany.
¹³ Data Science Lab, Office of the Chief Information Officer, Smithsonian Institution, Washington, DC.

PMID: 36807678
PMCID: PMC9985156
DOI: 10.1093/gbe/evad024

A Chromosome-length Assembly of the Black Petaltail (Tanypteryx hageni) Dragonfly

Ethan R Tolman et al. Genome Biol Evol. 2023.

. 2023 Mar 3;15(3):evad024.

doi: 10.1093/gbe/evad024.

Authors

Affiliations

¹ American Museum of Natural History, Department of Invertebrate Zoology, New York, New York.
² The City University of New York Graduate Center, New York, New York.
³ Program for Conservation Genomics, Department of Biology, Stanford University.
⁴ Department of Plant and Wildlife Sciences, Brigham Young University, Provo, Utah.
⁵ Department of Natural Sciences, Baruch College, New York, New York.
⁶ Department of Microbiology and Molecular Biology, Brigham Young University, Provo, Utah.
⁷ The Center for Genome Architecture, Department of Molecular and Human Genetics, Baylor College of Medicine, Houston, Texas.
⁸ The University of Western Australia, UWA School of Agriculture and Environment, Crawley, Washington, Australia.
⁹ Center for Theoretical Biological Physics and Department of Computer Science, Rice University, Houston, Texas.
¹⁰ Broad Institute of MIT and Harvard, Cambridge, Massachusetts.
¹¹ Shanghai Institute for Advanced Immunochemical Studies, ShanghaiTech, Pudong, China.
¹² LOEWE Centre for Translational Biodiversity Genomics (LOEWE-TBG), Frankfurt, Germany.
¹³ Data Science Lab, Office of the Chief Information Officer, Smithsonian Institution, Washington, DC.

PMID: 36807678
PMCID: PMC9985156
DOI: 10.1093/gbe/evad024

Abstract

We present a chromosome-length genome assembly and annotation of the Black Petaltail dragonfly (Tanypteryx hageni). This habitat specialist diverged from its sister species over 70 million years ago, and separated from the most closely related Odonata with a reference genome 150 million years ago. Using PacBio HiFi reads and Hi-C data for scaffolding we produce one of the most high-quality Odonata genomes to date. A scaffold N50 of 206.6 Mb and a single copy BUSCO score of 96.2% indicate high contiguity and completeness.

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Figures

**Fig. 1.**
(A) *T. hageni*, adult. E. HiC contact map with the chromosome level assembly. (B) Phylogeny of Odonata (modified from Kohli et al. 2021). Families with a reference genome (The zygopterya Cholorcyphidae, Calopterygidae, Platycnemididae and Anisoptera Libellulidae) highlighted in purple, Petaluridae highlighted in green. (C) *T. hageni*, larvae, credit Marla Garrison. (D) A fen in Lassen National Forest (California, USA) where *T. hageni* was collected. (Upper right) E. Hi-C contact map visualizing the alignment to the reference genome assembly.

See this image and copyright information in PMC

References

1. Allio R, et al. . 2020. Mitofinder: efficient automated large-scale extraction of mitogenomic data in target enrichment phylogenomics. Mol Ecol Resour 20:892–905. - PMC - PubMed
1. Ardila-Garcia AM, Gregory TR. 2009. An exploration of genome size diversity in dragonflies and damselflies (Insecta: Odonata). J Zool 278:163–173.
1. Baird I. 2012. The wetland habitats, biogeography and population dynamics of Petalura gigantea (Odonata: Petaluridae) in the Blue Mountains of New South Wales. PhD, The University of Western Sydney.
1. Bedford BL, Godwin KS. 2003. Fens of the United States: distribution, characteristics, and scientific connection versus legal isolation. Wetlands 23:608–629.
1. Boulesteix M, Weiss M, Biémont C. 2006. Differences in Genome Size Between Closely Related Species: The Drosophila melanogaster Species Subgroup. Mol Biol Evol 23:162–167. doi:10.1093/molbev/msj012 - DOI - PubMed

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A Chromosome-length Assembly of the Black Petaltail (Tanypteryx hageni) Dragonfly

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A Chromosome-length Assembly of the Black Petaltail (Tanypteryx hageni) Dragonfly

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