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. 2023:2615:219-228.
doi: 10.1007/978-1-0716-2922-2_16.

Studying Mitochondrial Nucleic Acid Synthesis Utilizing Intact Isolated Mitochondria

Jelena Misic  1 , Dusanka Milenkovic  2
Affiliations

Studying Mitochondrial Nucleic Acid Synthesis Utilizing Intact Isolated Mitochondria

Jelena Misic et al. Methods Mol Biol. 2023.

Abstract

Mitochondria are eukaryotic organelles of endosymbiotic origin that contain their own genetic material, mitochondrial DNA (mtDNA), and dedicated systems for mtDNA maintenance and expression. MtDNA molecules encode a limited number of proteins that are nevertheless all essential subunits of the mitochondrial oxidative phosphorylation system. Here, we describe protocols to monitor DNA and RNA synthesis in intact, isolated mitochondria. These in organello synthesis protocols are valuable techniques for studying the mechanisms and regulation of mtDNA maintenance and expression.

Keywords: Mitochondria; Radioactive labeling of nucleic acids; in organello replication and transcription; mtDNA; mtDNA maintenance and expression.

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References

    1. Gustafsson CM, Falkenberg M, Larsson N-G (2016) Maintenance and expression of mammalian mitochondrial DNA. Annu Rev Biochem 85:133–160 - DOI - PubMed
    1. Protasoni M, Zeviani M (2021) Mitochondrial structure and bioenergetics in normal and disease conditions. Int J Mol Sci 22:586 - DOI - PubMed - PMC
    1. Pfanner N, Warscheid B, Wiedemann N (2019) Mitochondrial proteins: from biogenesis to functional networks. Nat Rev Mol Cell Biol 1:267–284
    1. Viscomi C, Zeviani M (2017) MtDNA-maintenance defects: syndromes and genes. J Inherit Metab Dis 40:587–599 - DOI - PubMed - PMC
    1. Falkenberg M (2018) Mitochondrial DNA replication in mammalian cells: overview of the pathway. Essays Biochem 62:287–296 - DOI - PubMed - PMC

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