Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023;95(2):e10843.
doi: 10.1002/wer.10843.

Monitoring hospital effluents through physico-chemical characterization and genotoxic testing

Priyanka Mathur  1 , Kusum Rani, Pradeep Bhatnagar  1
Affiliations

Monitoring hospital effluents through physico-chemical characterization and genotoxic testing

Priyanka Mathur et al. Water Environ Res. 2023.

Abstract

Inefficient monitoring of the water resource recovery facility at various health care establishments may lead to detrimental effects on human population, especially when such water get mixed with potable municipal water supply. To ensure the efficiency and proper functioning of the water resource recovery facility and quality of water, before it being disposed of, the present study was conducted with the assessment of physico-chemical parameters of water and its genotoxic and cytogenetic effects in mice. The sample water was given ad libitum to animals for three different time durations, that is, 7, 15, and 30 days. Bone marrow chromosomal aberration and bone marrow micronucleus (MN) assay were performed to get the extent of genotoxicity and cytogenicity. The results showed presence of chromosomal aberrations like fragments breaks and ring formation in different groups. Besides this, a significant (p < 0.05*, p < 0.01**, p < 0.001***) decrease in the mitotic index was noted in the group which was given 100% concentrated sample water for 30 days. A significant (p < 0.05*, p < 0.01**, p < 0.001***) increase in MN induction and decreased ratio of polychromatic to normochromatic erythrocytes were observed in groups which were given samples at 10% and 100% concentrations for longer durations. The findings suggest that even the recovered sample water reflects a positive in vivo genotoxic potential when given for a longer duration of 30 days, indicating some lacuna in the treatment process.

Keywords: chromosomal aberration assay; environmental health; genotoxic assessment; liquid wastewater testing; micronucleus assay; physico-chemical characterization.

PubMed Disclaimer

References

REFERENCES

    1. Alabi, O. A., & Shokunbi, O. S. (2011). Toxicological effects of hospital wastewater using animal bioassays. Annals of Biological Research, 2(2), 265-275. https://www.researchgate.net/publication/264537405_Toxicological_effects...
    1. APHA method 3111: Metals by Flame Atomic Absorption Spectrometery. (1992). Standard methods for the examination of water and wastewater (18th ed.). American Public Health Association. https://law.resource.org/pub/us/cfr/ibr/002/apha.method.3111.1992.html
    1. APHA-AWWA-WEF. (2005). Standard methods for the examination of water and wastewater (21st ed.). American Public Health Association. https://www.worldcat.org/title/standard-methods-for-the-examination-of-w...
    1. Ben, Y., Fu, C., Hu, M., Liu, L., Wong, M. H., & Zheng, C. (2019). Human health risk assessment of antibiotic resistance associated with antibiotic residues in the environment: A review. Environmental Research, 169, 483-493. https://doi.org/10.1016/j.envres.2018.11.040
    1. Bhat, S. A., Hassan, T., & Majid, S. (2019). Heavy metal toxicity and their harmful effects on living organisms-A review. International Journal of Medical Science and Diagnosis Research, 3(1), 106-122. https://doi.org/10.32553/JMSDR

LinkOut - more resources