Molecularly imprinted sensor based on poly-o-phenylenediamine-hydroquinone polymer for β-amyloid-42 detection

Abstract

A sensitive and selective molecularly imprinted polymer (MIP) sensor was developed for the determination of amyloid-β (1-42) (Aβ₄₂). The glassy carbon electrode (GCE) was successively modified with electrochemical reduction graphene oxide (ERG) and poly(thionine-methylene blue) (PTH-MB). The MIPs were synthesized by electropolymerization with Aβ₄₂ as a template and o-phenylenediamine (o-PD) and hydroquinone (HQ) as functional monomers. Cyclic voltammetry (CV), electrochemical impedance spectroscopy (EIS), chronoamperometry (CC), and differential pulse voltammetry (DPV) were used to study the preparation process of the MIP sensor. The preparation conditions of the sensor were investigated in detail. In optimal experimental conditions, the response current of the sensor was linear in the range of 0.12-10 μg mL^-1 with a detection limit of 0.018 ng mL^-1. The MIP-based sensor successfully detected Aβ₄₂ in commercial fetal bovine serum (cFBS) and artificial cerebrospinal fluid (aCSF).

Keywords: Amyloid-β (1–42); Differential pulse voltammetry; Electrochemically reduced graphene oxide; Molecularly imprinted sensor; Poly(thionine-methylene blue).