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. 2023 Feb 16:16:961-976.
doi: 10.2147/IDR.S395220. eCollection 2023.

Molecular Epidemiology of Staphylococcus aureus in a Tertiary Hospital in Anhui, China: ST59 Remains a Serious Threat

Huimin Zhang  1 , Jiaxin Cao  1 , Zhien He  2   3 , Xianchun Zong  1 , Baolin Sun  2   3
Affiliations

Molecular Epidemiology of Staphylococcus aureus in a Tertiary Hospital in Anhui, China: ST59 Remains a Serious Threat

Huimin Zhang et al. Infect Drug Resist. .

Abstract

Purpose: This study aimed to investigate the molecular characteristics, antimicrobial resistance and hemolytic phenotype of Staphylococcus aureus isolated from Anhui, China.

Results: From August 2021 to January 2022, 214 S. aureus isolates were collected from the Anhui Provincial Hospital. This study identified 117 methicillin-resistant S. aureus and 97 methicillin-sensitive S. aureus isolates, and the detection rate of methicillin-resistant isolates was 1.8-fold higher than the average isolates reported in China (53.9% vs 30.5%). S. aureus isolates share identity at five or more of the seven MLST-based housekeeping loci, referred to as the clonal complex (CC). Forty ST types were found in 214 clinical S. aureus isolates, with the most extensive distribution of ST59 and ST6697 typing numbers and higher CC5 detection rates than any other clonal group. (The ST typing is the result of the MLST typing website query.) To detect the virulence of ST types of S. aureus, hemolysis experiments were performed on 214 clinical isolates, and it was concluded that ST59 had a relatively robust hemolytic capacity.

Conclusion: Anhui S. aureus isolates have unique molecular and antibiotic resistance profiles. The antibiotic resistance profile may be related to the random use of antibiotics.

Keywords: Staphylococcus aureus; epidemiological analysis; hemolytic; multilocus sequence typing; phenotype.

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Conflict of interest statement

The authors have no conflicts of interest to declare in this work.

Figures

Figure 1
Figure 1
The distribution of sample sources.
Figure 2
Figure 2
Distribution of MRSA and MSSA in different departments (n>3) (**Indicates significant level).
Figure 3
Figure 3
2015–2020 MRSA detection rate and intensity of antimicrobial drug use.
Figure 4
Figure 4
The part between cloning complex (CC) and ST types.
Figure 5
Figure 5
Minimum spanning tree for ST types Minimum spanning tree generated from fractal data using the phyloviz website (http://online.phyloviz.net/index). Each sphere or node represents a unique ST type. The size of each node indicates the number of S. aureus isolates of each ST type. The length between two nodes reflects the genetic distance between the two bounding ST types.
Figure 6
Figure 6
Hemolysis box diagram of different subtypes (A): ST1, ST5, ST6, ST7, ST8, ST15, ST22, ST25; (B) ST59, ST72, ST88, ST188, ST239, ST630, ST764, ST5529, ST6697).
None
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