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. 2023 Feb 1;8(6):6009-6015.
doi: 10.1021/acsomega.2c07960. eCollection 2023 Feb 14.

LncRNA-GAS5 and β-Catenin as Independent Predictors of Asymptomatic Organ Damage in Nondiabetic Hypertensive Patients

Marwa M Esawy  1 , Amir Abd-Elhameed  2 , Ahmed F Gomaa  2 , Shereen A Baioumy  3 , Moataz A ElKot  4 , Mohammed A Hegab  5 , Ahmed S Alazzouni  6 , Felwa A Thagfan  7 , Rewaida Abdel-Gaber  8 , Mohamed A Dkhil  6   9 , Marwa A Shabana  1
Affiliations

LncRNA-GAS5 and β-Catenin as Independent Predictors of Asymptomatic Organ Damage in Nondiabetic Hypertensive Patients

Marwa M Esawy et al. ACS Omega. .

Abstract

Hypertension is a serious medical condition that can increase the risk of developing heart, brain, kidney, and other diseases. Many asymptomatic hypertension patients experience asymptomatic organ damage (AOD). The purpose of this study was to determine the roles of LncRNA-GAS5 and β-catenin in predicting AOD in hypertensive nondiabetic patients. This study included 256 subjects, 128 hypertension patients (75 of whom had AOD, and 53 of whom did not) and 128 healthy controls. qRT-PCR was used to assess LncRNA-GAS5, and ELISA was used to assess β-catenin. The LncRNA-GAS5 expression level was decreased in hypertensive patients compared to controls (p-value < 0.001). On the other hand, β-catenin levels showed higher levels in the patients in comparison with controls (p-value < 0.001). A 0.38-fold change in LncRNA-GAS5 expression predicted AOD with 86.6% sensitivity and 88.7% specificity. β-Catenin > 80.5 pg/mL predicted AOD with a sensitivity of 82.6% and specificity of 69.8%. LncRNA-GAS5 expression was a better diagnostic predictor of AOD than β-catenin. According to multivariate logistic regression analysis, decreased LncRNA-GAS5 expression independently increased the risk of AOD (adjusted odds ratio = 0.03 (95% CI: 0.01-0.1) (p < 0.001). Furthermore, elevated β-catenin levels may be an independent risk factor for AOD (adjusted odds ratio = 14.3 (95% confidence interval, 3.3-61.9) (p < 0.001). Collectively, in hypertensive patients, LncRNA GAS5 and β-catenin can distinguish patients with AOD from those who do not have AOD. LncRNA GAS5 and β-catenin can be used as independent predictors of AOD in hypertensive patients.

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Conflict of interest statement

The authors declare no competing financial interest.

Figures

Figure 1
Figure 1
Study flowchart.
Figure 2
Figure 2
Real-time RT-PCR analysis of lncRNA-GAS5 and β-catenin. Signals for genes of interest were normalized to GAPDH signals, and relative expression is given as a fold increase compared to control.
Figure 3
Figure 3
ROC curve analysis of the prediction of AOD.
See this image and copyright information in PMC

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