Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb;29(2):e13253.
doi: 10.1111/srt.13253.

Fractional radiofrequency-assisted delivery of fluorescent tracer dyes in ex vivo and in vivo skin: A proof-of-concept investigation using confocal microscopy

Gabriela Lladó Grove  1 , Rikke Louise Christensen  1 , Merete Haedersdal  1 , Vinzent Kevin Ortner  1
Affiliations

Fractional radiofrequency-assisted delivery of fluorescent tracer dyes in ex vivo and in vivo skin: A proof-of-concept investigation using confocal microscopy

Gabriela Lladó Grove et al. Skin Res Technol. 2023 Feb.
No abstract available

Keywords: confocal microscopy; drug delivery; energy-based device; ex vivo porcine skin; fluorescence microscopy; fluorescence trace dye; fractional radiofrequency; in vivo human skin; skin remodeling; tissue interaction.

PubMed Disclaimer

Conflict of interest statement

MH has received equipment and research grant from Venus Concept Inc. Other authors have no conflict of interest to declare.

Figures

FIGURE 1
FIGURE 1
Confocal microscopy images of ex vivo porcine and in vivo human skin exposed to fractional radiofrequency (FRF) pretreatment and fluorescent tracer dyes. In ex vivo porcine skin (columns 1 and 2 from the left) combined RCM + FCM demonstrates superficial damage in digitally‐stained AO‐labeled images and a focal increase of ATTO‐647N in the MTZ. In in vivo human skin (columns 3 and 4 from the left), the LSRs and MTZ‐grid pattern are visualized clinically and with RCM + FCM, and the horizontal diffusion of NaFl is shown in the FRF‐treated skin sites at a tissue depth of 24 μm. AO, acridine orange; FCM, fluorescence confocal microscopy; LSR, local skin reaction; MTZ, microthermal zone; NaFl, sodium fluorescein; RCM, reflectance confocal microscopy
See this image and copyright information in PMC

Similar articles

See all similar articles

References

    1. Wenande E, Anderson RR, Haedersdal M. Fundamentals of fractional laser‐assisted drug delivery: an in‐depth guide to experimental methodology and data interpretation. Adv Drug Deliv Rev. 2020;153:169‐184. doi:10.1016/j.addr.2019.10.003 - DOI - PubMed
    1. Foged C, Haedersdal M, Bik L, Dierickx C, Phillipsen PA, Togsverd‐Bo K. Thermo‐mechanical fractional injury enhances skin surface‐ and epidermis‐protoporphyrin IX fluorescence: comparison of 5‐aminolevulinic acid in cream and gel vehicles. Lasers Surg Med. 2021;53(5):622‐629. doi:10.1002/lsm.23326 - DOI - PubMed
    1. Banzhaf CA, Ortner VK, Philipsen PA, Haedersdal M. The ablative fractional coagulation zone influences skin fluorescence intensities of topically applied test molecules—an in vitro study with fluorescence microscopy and fluorescence confocal microscopy. Lasers Surg Med. 2019;51(1):68‐78. doi:10.1002/lsm.23034 - DOI - PubMed
    1. Eubanks SW, Solomon JA. Safety and efficacy of fractional radiofrequency for the treatment and reduction of acne scarring: a prospective study. Lasers Surg Med. 2022;54(1):74‐81. doi:10.1002/lsm.23453 - DOI - PMC - PubMed
    1. Hongcharu W, Gold M. Expanding the clinical application of fractional radiofrequency treatment: findings on rhytides, hyperpigmentation, rosacea, and acne redness. J Drugs Dermatol. 2015;14(11):1298‐1304. http://www.ncbi.nlm.nih.gov/pubmed/26580880 - PubMed

Substances