Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Feb 14:2023:5956951.
doi: 10.1155/2023/5956951. eCollection 2023.

Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients

Yan Zhang  1 , Xiangdi Meng  2 , Zhaosen Ma  2 , Zhou Sun  2 , Zhixin Wang  2
Affiliations

Association of Androgen-Receptor Gene Mutations with the Copy Number of Androgen-Receptor Silk Protein A Complex and Glutathione-S-Transferases T1 and M1 in Prostate Cancer Patients

Yan Zhang et al. Genet Res (Camb). .

Abstract

Objective: The purpose of our work was to explore the association of mutations in the androgen receptor gene and copy numbers of the androgen-receptor silk protein A complex with glutathione-S-transferases T1 and M1 in prostate cancer patients.

Materials and methods: Eighty-five patients with PC and 85 healthy controls were included in the study. Fasting peripheral venous blood was collected, whole blood genomic DNA was extracted, and AR gene-receptor genotype was detected by a high-resolution melting curve analysis detection technology. Expression levels of androgen receptor (AR) and filamin protein A (FlnA) were detected by Western blotting. RT-PCR was used to detect the copy number of T1 and M1 glutathione-S-transferases.

Results: The wild-type androgen receptor gene rs5918762 is of TT type. The frequencies of CC and TC genes in the prostate cancer group were significantly higher than those in the normal control group (P < 0.05). Compared with TT-type PC patients, PC patients with TC-type and CC-type had higher expression levels of sex hormone receptor silk protein A complex and higher copy numbers of GSTT1 and GSTM1 (P < 0.05). Androgen-receptor gene mutation (T ⟶ C) was significantly positively correlated with the expression level of androgen-receptor silk protein A complex and the copy number of GSTT1 and GSTM1.

Conclusion: Androgen-receptor gene polymorphisms were significantly associated with expression levels of androgen receptor complex A and silk proteins, and copy numbers of T1 and M1 glutathione-S-transferases. A combination of four factors can be used to identify prostate cancer susceptibility and disease progression.

PubMed Disclaimer

Conflict of interest statement

The authors declare that they have no conflicts of interest.

Figures

Figure 1
Figure 1
MRI image of prostate in patients with prostate cancer.
Figure 2
Figure 2
Expression of androgen receptor silk protein A complex. Note. 1–3 are samples of the normal control group; 4–6 are samples of the prostate cancer group.
See this image and copyright information in PMC

Similar articles

See all similar articles

Cited by

References

    1. Spratt D. E., Malone S., Roy S., et al. Prostate radiotherapy with adjuvant androgen deprivation therapy (ADT) improves metastasis-free survival compared to neoadjuvant ADT: an individual patient meta-analysis. Journal of Clinical Oncology . 2021;39(2):136–144. doi: 10.1200/jco.20.02438. - DOI - PMC - PubMed
    1. Aurilio G., Cimadamore A., Mazzucchelli R., et al. Androgen receptor signaling pathway in prostate cancer: from genetics to clinical applications. Cells . 2020;9(12):p. 2653. doi: 10.3390/cells9122653. - DOI - PMC - PubMed
    1. Di Donato M., Giovannelli P., Barone M. V., Auricchio F., Castoria G., Migliaccio A. A small peptide targeting the ligand-induced androgen receptor/filamin a interaction inhibits the invasive phenotype of prostate cancer cells. Cells . 2021;11(1):p. 14. doi: 10.3390/cells11010014. - DOI - PMC - PubMed
    1. Barul C., Richard H., Parent M. E. Night-shift work and risk of prostate cancer: results from a Canadian case-control study, the prostate cancer and environment study. American Journal of Epidemiology . 2019;188(10):1801–1811. doi: 10.1093/aje/kwz167. - DOI - PMC - PubMed
    1. Benabdelkrim M., Djeffal O., Berredjem H. GSTM1 and GSTT1 polymorphisms and susceptibility to prostate cancer: a case-control study of the Algerian population. Asian Pacific Journal of Cancer Prevention . 2018;19(10):2853–2858. doi: 10.22034/APJCP.2018.19.10.2853. - DOI - PMC - PubMed