Skip to main page content
U.S. flag

An official website of the United States government

Dot gov

The .gov means it’s official.
Federal government websites often end in .gov or .mil. Before sharing sensitive information, make sure you’re on a federal government site.

Https

The site is secure.
The https:// ensures that you are connecting to the official website and that any information you provide is encrypted and transmitted securely.

Access keys NCBI Homepage MyNCBI Homepage Main Content Main Navigation
. 2023 Dec;38(12):1403-1413.
doi: 10.14670/HH-18-595. Epub 2023 Feb 20.

MiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian cancer

Zhanyu Wang  1 , Xiaoping Zhang  1 , Yongying Liu  1 , Xiaoyan Shi  1 , Lijun Li  1 , Yun Jia  1 , Fangfang Wu  1 , Haosen Cui  1 , Liang Li  2
Affiliations

MiR-5195-3p functions as a tumor suppressor by targeting RHBDD1 in ovarian cancer

Zhanyu Wang et al. Histol Histopathol. 2023 Dec.

Abstract

Background: Recent studies have reported the tumor suppressive role of miR-5195-3p in the progression of several cancers, but the potential roles of miR-5195-3p in ovarian cancer (OC) remain largely unknown.

Methods: We first analyzed the expression levels of miR-5195-3p in 83 pairs of human OC tissues and adjacent specimens by reverse transcription-quantitative PCR. The correlation of miR-5195-3p/rhomboid domain containing 1 (RHBDD1) and clinicopathological parameters was analyzed by chi-square test. The prognostic value of miR-5195-3p was evaluated by Kaplan-Meier method Cox proportional hazards models. The effects of miR-5195-3p on cell proliferation, cell cycle distribution, migration and invasion were examined by CCK-8 assay, colony formation assay, flow cytometry and transwell assay. Tumor forming was evaluated by nude mice model in vivo. The association between miR-5195-3p and RHBDD1 was verified by luciferase reporter assay.

Results: We observed that miR-5195-3p level was remarkably reduced in OC tissues as compared to adjacent tissues. The expression of miR-5195-3p was associated with FIGO stage, depth of invasion and poor survival prognosis in OC patients. Overexpression of miR-5195-3p significantly suppressed cell proliferation, cell cycle G1/S transition, migration and invasion in OC cell lines (SKOV-3 and OVCAR3), while knockdown of miR-5195-3p obtained the opposite results. We further confirmed miR-5195-3p as a negative post-transcriptional modulator of RHBDD1. RHBDD1 expression was upregulated in OC tissues compared with adjacent tissues, which was inversely correlated with miR-5195-3p expression. The expression of RHBDD1 was associated with FIGO stage and distant metastasis. RHBDD1 overexpression reversed the suppressive role of miR-5195-3p on OC cell proliferation, migration and invasion. Consistent with the in vitro results, miR-5195-3p overexpression decreased the growth of subcutaneously inoculated tumors in nude mice.

Conclusions: Taken together, the present results indicated that miR-5195-3p acts a tumor suppressor by targeting RHBDD1 in OC.

PubMed Disclaimer

References

    1. Armstrong D.K., Alvarez R.D., Bakkum-Gamez J.N., Barroilhet L., Behbakht K., Berchuck A., Chen L.M., Cristea M., DeRosa M., Eisenhauer E.L., Gershenson D.M., Gray H.J., Grisham R., Hakam A., Jain A., Karam A., Konecny G.E., Leath C.A., Liu J., Mahdi H., Martin L., Matei D., McHale M., McLean K., Miller D.S., O'Malley D.M., Percac-Lima S., Ratner E., Remmenga S.W., Vargas R., Werner T.L., Zsiros E., Burns J.L. and Engh A.M. (2021). Ovarian cancer, version 2.2020, NCCN clinical practice guidelines in oncology. J. Natl. Compr. Canc. Netw. 19, 191-226. - PubMed
    1. Boussios S., Mikropoulos C., Samartzis E., Karihtala P., Moschetta M., Sheriff M., Karathanasi A., Sadauskaite A., Rassy E. and Pavlidis N. (2020). Wise management of ovarian cancer: On the cutting edge. J. Pers. Med. 10, 41. - PMC - PubMed
    1. Bracken C.P., Scott H.S. and Goodall G.J. (2016). A network-biology perspective of microRNA function and dysfunction in cancer. Nat. Rev. Genet. 17, 719-732. - PubMed
    1. Ebrahimi S.O. and Reiisi S. (2019). Downregulation of miR-4443 and miR-5195-3p in ovarian cancer tissue contributes to metastasis and tumorigenesis. Arch. Gynecol. Obstet. 299, 1453-1458. - PubMed
    1. Freeman M. (2008). Rhomboid proteases and their biological functions. Annu. Rev. Genet. 42, 191-210. - PubMed

LinkOut - more resources